EFFECTS OF CONCANAVALIN A ON MOUSE PERITONEAL MACROPHAGES
EFFECTS OF CONCANAVALIN A ON MOUSE PERITONEAL MACROPHAGES
复制标题
刀豆蛋白 A 对小鼠腹腔巨噬细胞的影响
DOI：
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发表时间：
1974
影响因子：
15.3
通讯作者：
Z. Cohn
中科院分区：
文献类型：
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作者：
P. Edelson;Z. Cohn
伴刀豆球蛋白A（ConA）与小鼠腹腔巨噬细胞质膜上的糖残基结合，并刺激膜的广泛胞饮化。通过加入Con A，总的胞饮速率增加3.5-4.5倍，并且表面标记酶腺苷三磷酸酶可以与细胞暴露于Con A后产生的胞质囊泡相关联地进行组织化学鉴定。一旦形成，这些胞饮小泡可以持续数天，并且不能显示与初级或预先形成的次级溶酶体融合的形态学证据。与Con A同时或随后给药，对巨噬细胞摄取乳胶颗粒或IgG包被SRBC的能力无明显影响。
Concanavalin A (Con A) binds to saccharide residues on the mouse peritoneal macrophage plasma membrane and stimulates extensive pinocytic interiorization of the membrane. The overall pinocytic rate is increased 3.5–4.5 times by the addition of Con A, and the surface marker enzyme adenosine triphosphatase can be identified histochemically in association with the cytoplasmic vesicles generated after exposure of the cells to Con A. Once formed, these pinocytic vesicles may persist for several days and fail to show morphologic evidence of fusion with primary or preformed secondary lysosomes. There is no apparent effect on the capacity of the macrophage to ingest either latex particles or IgG-coated SRBC administered either simultaneously with or subsequent to the Con A.