On the integrity of a commercial cassette ultrafiltration membrane: implications for marine colloidal biogeochemistry
On the integrity of a commercial cassette ultrafiltration membrane: implications for marine colloidal biogeochemistry
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商业盒式超滤膜的完整性：对海洋胶体生物地球化学的影响
DOI：
10.1007/s13131-014-0521-7
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发表时间：
2014-09
影响因子：
1.4
通讯作者：
Min Chen
中科院分区：
文献类型：
--
作者：
Liangshi Lin;Yihua Cai;Xiuwu Sun;Min Chen
用一套不同分子质量的大分子测试了盒式交叉流超过滤器（Pellicon 2, Millipore）的性能和完整性。交叉流超滤实验中，随着分子质量的增加，保留系数增大，在ml - q水和超滤海水介质中，当葡聚糖为10 kDa时，保留系数均达到90%。基于90%的保留系数，超滤膜的分子质量截止值定义为10 kDa，这是制造商额定的10倍（1 kDa）。为了进一步验证实验室标定的准确性，采用盒式超滤膜对珠江下游和九龙江口的样品进行了超滤，并采用基于时间序列渗透子样品的超滤渗透模型对样品中的胶体有机碳丰度进行了定量。胶体有机碳丰度在九龙江河口为5.8% ~ 21.1%，珠江下游为5.6% ~ 11.0%。这与两个河口的报告值以及沿海和开放海洋中具有10 kDa截止膜的海洋环境中的胶体有机碳丰度一致。因此，这些现场数据支持实验室校准结果，并表明所采用的实验和量化程序的有效性。在胶体和纳米粒子生物地球化学研究中，超滤膜的标称分子质量截止值与实际孔径之间的差异应引起高度关注。在超滤实验中应仔细检查膜的完整性，以避免误导分子质量截止信息。
The performance and integrity of a cassette cross-flow ultrafilter (Pellicon 2, Millipore) are examined with a suite of macromolecules of different molecular masses. The retention coefficient during the cross-flow ultrafiltration experiments increases with increasing molecular mass and reaches 90% with 10 kDa dextran in both milli-Q water and ultrafiltered seawater media. Based on a 90% retention coefficient, the molecular mass cut-off for the ultrafiltration membrane is defined at 10 kDa, which is ten times (1 kDa) that rated by the manufacturer. To further validate the accuracy of the laboratory calibration, the samples from the lower Zhujiang River and the Jiulong River Estuary are ultrafiltered with the cassette ultrafiltration membrane and the colloidal organic carbon abundances in these samples are quantified with the ultrafiltration permeation model based on time series permeation subsamples. The colloidal organic carbon abundances are 5.8%–21.1% in the Jiulong River Estuary and 5.6%–11.0% in the lower Zhujiang River. These are consistent with the reported values for both estuaries as well as with the colloidal organic carbon abundances in marine environments over the coastal and open oceans with 10 kDa cut-off membranes. Therefore, these field data support the laboratory calibration result and indicate the validity of the experimental and quantification procedure adopted. The discrepancy between the nominal molecular mass cut-off and the actual pore size of the ultrafiltration membrane should be of great concern for research in colloidal and nanoparticle biogeochemistry. Careful examination of the membrane integrity should be taken during ultrafiltration experiments in order to avoid misleading molecular mass cut-off information.
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影响因子：
3
作者：
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通讯作者：
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影响因子：
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作者：
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通讯作者：
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