A cyclic nucleotide-gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster
A cyclic nucleotide-gated channel in the brain regulates olfactory modulation through neuropeptide F in fruit fly Drosophila melanogaster
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DOI：
10.1002/arch.21620
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发表时间：
2019-10-17
影响因子：
2.2
通讯作者：
Kim, Do-Hyoung
中科院分区：
文献类型：
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作者：
Lee, Sion;Jones, Walton D.;Kim, Do-Hyoung
嗅觉感知及其调节对于昆虫识别环境中的各种气味并做出正确的生存决定非常重要。识别参与嗅觉调节的新基因并揭示其机制可能会让我们了解中枢神经系统的决策过程。在这里，我们报告了环核苷酸门控（CNG）通道CG42260在调节ab3A嗅觉感觉神经元中的一种新的嗅觉功能，该神经元对果蝇果蝇的食物源气味做出特异性反应。我们发现两个独立的 CG42260 突变体在 ab3A 神经元中表现出反应减弱。与哺乳动物 CNG 不同，CG42260 不在气味感觉神经元中表达，而是广泛在中枢神经系统中表达，包括神经肽生成细胞。通过使用分子遗传学工具，我们在一对神经肽 F (NPF) 阳性 L1-1 细胞中确定了 CG42260 的表达，已知这些细胞可调节食物气味反应。 NPF神经元中CG42260的敲低减少了L1-1细胞中NPF的产生，这反过来导致ab3A神经元的神经元反应减少。我们的研究结果表明 CG42260 通过 NPF 调节对食物气味的嗅觉反应的新生物学功能。
Olfactory sensing and its modulation are important for the insects in recognizing diverse odors from the environment and in making correct decisions to survive. Identifying new genes involved in olfactory modulation and unveiling their mechanisms may lead us to understand decision making processes in the central nervous system. Here, we report a novel olfactory function of the cyclic nucleotide-gated (CNG) channel CG42260 in modulating ab3A olfactory sensory neurons, which specifically respond to food-derived odors in fruit fly Drosophila melanogaster. We found that two independent CG42260 mutants show reduced responses in the ab3A neurons. Unlike mammalian CNGs, CG42260 is not expressed in the odorant sensory neurons but broadly in the central nervous system including neuropeptide-producing cells. By using molecular genetic tools, we identified CG42260 expression in one pair of neuropeptide F (NPF) positive L1-l cells known to modulate food odor responsiveness. Knockdown of CG42260 in the NPF neurons reduced production of NPF in Ll-1 cells, which in turn, led to reduction of neuronal responses of the ab3A neurons. Our findings show the novel biological function of CG42260 in modulating olfactory responses to food odor through NPF.