Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging
Longitudinal imaging of Caenorhabditis elegans in a microfabricated device reveals variation in behavioral decline during aging
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DOI：
10.7554/elife.26652
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发表时间：
2017-05-31
期刊：
影响因子：
7.7
通讯作者：
Fang-Yen, Christopher
中科院分区：
文献类型：
--
作者：
Churgin, Matthew A.;Jung, Sang-Kyu;Fang-Yen, Christopher
蛔虫C.由于其寿命短和易于操纵的遗传学，秀丽线虫是衰老研究的支柱。目前，广泛使用的长期测量C。秀丽线虫受到低通量和难以进行老化表型的纵向监测的限制。在这里，我们描述了WorMotel，一种用于长期培养和自动纵向成像大量C。局限于单个威尔斯井中的秀丽线虫。使用WorMotel，我们发现短寿命和长寿命的菌株表现出行为衰退的模式，这些模式在个体或群体之间没有时间尺度，而是类似于野生型群体中最短和最长寿命的个体。我们还发现，蠕虫受氧化应激的行为轨迹类似于在衰老过程中观察到的轨迹。我们的方法是一个强大的和可扩展的工具，用于分析C。elegans行为和衰老。
The roundworm C. elegans is a mainstay of aging research due to its short lifespan and easily manipulable genetics. Current, widely used methods for long-term measurement of C. elegans are limited by low throughput and the difficulty of performing longitudinal monitoring of aging phenotypes. Here we describe the WorMotel, a microfabricated device for long-term cultivation and automated longitudinal imaging of large numbers of C. elegans confined to individual wells. Using the WorMotel, we find that short-lived and long-lived strains exhibit patterns of behavioral decline that do not temporally scale between individuals or populations, but rather resemble the shortest and longest lived individuals in a wild type population. We also find that behavioral trajectories of worms subject to oxidative stress resemble trajectories observed during aging. Our method is a powerful and scalable tool for analysis of C. elegans behavior and aging.