Differential effects of phenolic extracts from red-fleshed apple peels and flesh induced G1 cell cycle arrest and apoptosis in human breast cancer MDA-MB-231 cells
Differential effects of phenolic extracts from red-fleshed apple peels and flesh induced G1 cell cycle arrest and apoptosis in human breast cancer MDA-MB-231 cells
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红肉苹果皮和果肉中酚类提取物诱导人乳腺癌 MDA-MB-231 细胞 G1 细胞周期停滞和凋亡的不同作用
DOI：
10.1111/1750-3841.15863
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发表时间：
2021
影响因子：
3.9
通讯作者：
Chen Xue Sen
中科院分区：
文献类型：
--
作者：
Li Cui Xia;Lin Zhen Xian;Zhao Xian Hua;Zuo Wei Fang;Wang Nan;Zhang Zong Ying;Chen Xue Sen
摘要红肉苹果是首选，因为他们的高含量的酚类和抗氧化剂的果皮和果肉。在此，我们评估了来自新红肉苹果的苹果皮多酚提取物（APP）和苹果肉多酚提取物（AFP）抑制人乳腺癌MDA-MB-231细胞增殖和诱导细胞凋亡的机制。CCK-8法测定其抗增殖活性。Western blot检测蛋白表达。超高效液相色谱-串联质谱法测定了APP和AFP中14种主要酚类化合物的含量。此外，APP和AFP通过Akt途径诱导细胞凋亡，达到显著的抑制作用（显著上调活性氧[ROS]水平，下调pAkt、pBad、Bcl-2的表达，促进细胞色素c释放，激活Cle-Caspase 9，并诱导Cle-Caspase 3和Cle-PARP的表达），诱导G 0/G1期细胞阻滞（p-p53和p21表达增加，PCNA和Cyclin D1表达减少）。APP的抑制作用强于AFP。这些结果表明AFP和APP可能是治疗三阴性乳腺癌MDA‐MB‐231细胞的天然化学物质的良好来源。实际应用评价了红肉苹果皮和果肉酚类提取物对人乳腺癌MDA‐MB‐231细胞的抗增殖作用。这些数据可以明确红肉苹果的功能部位，为科研人员和消费者认识和开发红肉苹果提供一定的依据。
AbstractRed‐fleshedapples are preferredbecause of their high content of phenolics and antioxidants in peel and pulp. Herein, we evaluated the mechanisms of apple peel polyphenolic extracts (APP) and apple flesh polyphenolic extracts (AFP) from the new red‐fleshed apple in inhibiting cell proliferation and inducing apoptosis on human breast cancer MDA‐MB‐231 cells. The antiproliferative activities were determined by the CCK8 assay. The expression of proteins was determined using Western blot. We found that the content of polyphenols and flavonoids in APP was significantly higher than that in AFP, and 14 main phenolic compounds in APP and AFP were quantified using UPLC–MS/MS techniques. Besides, the significant inhibition effects of APP and AFP were achieved through Akt pathway by inducing apoptosis (significantly upregulating reactive oxygen species [ROS] levels, and downregulating expression of pAkt, pBad, Bcl‐2, promoting Cytochrome c release, activating Cle‐Caspase 9, and inducing expressions of Cle‐Caspase 3 and Cle‐PARP), and inducing G0/G1 cell cycle arrest (increased expressions of p‐p53 and p21 and decreased expressions of PCNA and Cyclin D1). And the inhibition effect of APP was stronger than that of AFP. These results suggest that AFP and APP may be excellent sources of natural chemicals for treating triple‐negative breast cancer MDA‐MB‐231 cells.Practical ApplicationThe effects of antiproliferation of phenolic extracts from red‐fleshed apple peels and flesh on human breast cancer MDA‐MB‐231 cells were evaluated. The data may clarify the functional parts of red‐fleshed apple and provide some basis for scientific researchers and consumers to recognize and exploit red‐fleshed apple.