Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves
Molecular cloning and characterization of a novel jasmonate inducible pathogenesis-related class 10 protein gene, JIOsPR10, from rice (Oryza sativa L.) seedling leaves
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DOI：
10.1006/bbrc.2001.5507
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发表时间：
2001-09-07
影响因子：
3.1
通讯作者：
Agrawal, VP
中科院分区：
文献类型：
--
作者：
Jwa, NS;Agrawal, GK;Agrawal, VP
从2周龄茉莉酸处理的水稻幼苗叶片制备的cDNA文库中克隆了一个与高粱发病机制相关的10类蛋白基因同源的新型水稻基因（Oryza sativa L.），并将其命名为JIOsPR10（茉莉酸诱导型）。 JIOsPR10 编码 160 个氨基酸的多肽，预测分子量为 17,173.23 Da，pI 为 5.84。 JIOsPR10 与高粱 PR10 蛋白高度相似（77%），但与其他已鉴定的 PR10 在氨基酸水平上的相似性不到 55%。基因组 Southern 分析表明水稻基因组中存在相关基因。在健康叶子中未检测到 JIOsPR10 转录物，并且在切割后也未诱导。进一步的表达分析表明，防御/应激途径的信号成分茉莉酸、水杨酸和 H2O2 相对于剪切对照显着上调 JIOsPR10 mRNA，而其他两种应激调节剂乙烯和脱落酸未能诱导其表达。有趣的是，蛋白磷酸酶 (PP) 抑制剂斑蝥素、内索索和冈田酸快速有效地上调 JIOsPR10 表达，表明参与了磷酸化/去磷酸化事件。此外，JIOsPR10 基因的诱导表达受到光信号的影响。最后，稻瘟病原菌（Magnaporthe grisea）还特异地引发了 JIOsPR10 mRNA 在叶子中的积累。信号分子、PP 抑制剂和病原体攻击诱导 JIOsPR10 基因表达，强烈表明了其作用。寻找水稻自卫/应激反应中的这种新基因。 (C) 2001 年学术出版社。
A novel rice (Oryza sativa L.) gene, homologous to a sorghum pathogenesis-related class 10 protein gene, was cloned from a cDNA library prepared from 2-week-old jasmonic acid-treated rice seedling leaves, and named as JIOsPR10 (Jasmonate inducible). JIOsPR10 encoded a 160-amino-acid polypeptide with a predicted molecular mass of 17,173.23 Da and a pI of 5.84. JIOsPR10 was highly similar (77%) to the sorghum PR10 protein, but showed less than 55% similarity with other identified PR10s at the amino acid level. Genomic Southern analyses indicated the presence of related genes in the rice genome. The JIOsPR10 transcript was not detected in the healthy leaves, and was not induced after cut. Further expression analysis revealed that the signaling components of defense/stress pathways, jasmonate, salicylate, and H2O2 significantly up-regulated the JIOsPR10 mRNA over the cut control, whereas two other stress regulators, ethylene and abscisic acid, failed to induce its expression. Interestingly the protein phosphatase (PP) inhibitors, cantharidin, endothall, and okadaic acid, rapidly and potently up-regulated the JIOsPR10 expression, suggesting involvement of the phosphorylation/dephosphorylation events. Additionally, the inducible expression of the JIOsPR10 gene was influenced by light signal(s). Finally, the blast pathogen (Magnaporthe grisea) also specifically elicited the accumulation of JIOsPR10 mRNA in leaves. Induction of the JIOsPR10 gene expression by signaling molecules, PP inhibitors and pathogen attack, strongly indicate a role. for this novel gene in rice self-defense/stress response(s). (C) 2001 Academic Press.