MACROPHAGES IN DROSOPHILA EMBRYOS AND L2 CELLS EXHIBIT SCAVENGER RECEPTOR-MEDIATED ENDOCYTOSIS
MACROPHAGES IN DROSOPHILA EMBRYOS AND L2 CELLS EXHIBIT SCAVENGER RECEPTOR-MEDIATED ENDOCYTOSIS
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DOI：
10.1073/pnas.89.21.10375
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发表时间：
1992-11-01
影响因子：
11.1
通讯作者：
KRIEGER, M
中科院分区：
文献类型：
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作者：
ABRAMS, JM;LUX, A;KRIEGER, M
哺乳动物巨噬细胞清道夫受体表现出异常广泛的结合特异性，并与动脉粥样硬化和宿主防御有关。在果蝇胚胎和原代胚胎细胞培养中观察到清道夫受体样内吞作用。这种受体活性主要由巨噬细胞表达。果蝇Schneider L2，但不是Kc，细胞系也表现出类似于其哺乳动物对应的清道夫受体介导的内吞途径。L2受体介导的高亲和力内化和随后的温度和氯喹敏感的降解I-125标记的乙酰化低密度脂蛋白和显示特征配体特异性。这些发现表明，清道夫受体介导重要的，保守的功能，并提高了它们可能是模式识别受体，在宿主防御机制的进化早期出现的可能性。他们还建立了额外的系统，用于研究果蝇的内吞作用和清道夫受体在疾病、宿主防御和发育中的功能。
Mammalian macrophage scavenger receptors exhibit unusually broad binding specificity and are implicated in atherosclerosis and host defense. Scavenger receptor-like endocytosis was observed in Drosophila melanogaster embryos and in primary embryonic cell cultures. This receptor activity was expressed primarily by macrophages. The Drosophila Schneider L2, but not the Kc, cell line also exhibited a scavenger receptor-mediated endocytic pathway similar to its mammalian counterpart. L2 receptors mediated high-affinity internalization and subsequent temperature- and chloroquine-sensitive degradation of I-125-labeled acetylated low density lipoprotein and displayed characteristic ligand specificity. These findings suggest that scavenger receptors mediate important, well-conserved functions and raise the possibility that they may be pattern recognition receptors that arose early in the evolution of host defense mechanisms. They also establish additional systems for the investigation of endocytosis in Drosophila and scavenger receptor function in disease, host defense, and development.