RAT OXYTOCIN RECEPTOR IN BRAIN, PITUITARY, MAMMARY-GLAND, AND UTERUS - PARTIAL SEQUENCE AND IMMUNOCYTOCHEMICAL LOCALIZATION
RAT OXYTOCIN RECEPTOR IN BRAIN, PITUITARY, MAMMARY-GLAND, AND UTERUS - PARTIAL SEQUENCE AND IMMUNOCYTOCHEMICAL LOCALIZATION
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DOI：
10.1210/en.136.9.4022
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发表时间：
1995-09-01
期刊：
影响因子：
4.8
通讯作者：
BURBACH, JPH
中科院分区：
文献类型：
--
作者：
ADAN, RAH;VANLEEUWEN, FW;BURBACH, JPH
从大鼠脑和子宫中克隆了催产素(OT)受体的部分互补DNA。这些互补的DNA编码相同的氨基酸序列，与人和猪的子宫OT受体高度同源，除了第三细胞内环的一个区域。针对这种大鼠OT受体的第三细胞内环的非重叠序列提出了抗体。使用这些抗血清，免疫细胞化学显示OT受体在脑、垂体、乳腺和子宫中的表达。在脑内，下丘脑腹内侧核、终纹床核、苍白球腹侧核、室旁核、视上核背侧等部位均有催产素受体免疫反应阳性。然而，通过受体放射自显影研究，在大脑的两个已知含有密集OT结合部位的区域：腹侧海马和杏仁中央核没有检测到免疫反应。在垂体中，前叶和后叶的OT受体免疫反应阳性，而中间叶为阴性。这些结果表明，相同的受体类型在外周OT靶组织和脑中都有表达，也表明在大脑的某些区域可能存在不同的OT受体亚型。
Partial complementary DNAs of an oxytocin (OT) receptor were cloned from rat brain and uterus. The complementary DNAs encoded for the same amino acid sequence, which showed a high degree of homology with the human and porcine uterine OT receptors, except for a region in the third intracellular loop. Antibodies were raised against nonoverlapping sequences of the third intracellular loop of this rat OT receptor. Using these antisera, OT receptor expression was demonstrated in the brain, pituitary, mammary gland, and uterus by immunocytochemistry. In the brain, several areas including the ventromedial hypothalamus, the bed nucleus of the stria terminalis, the ventral pallidum, the paraventricular nucleus, and the dorsal part of the supraoptic nucleus, demonstrated OT-receptor immunoreactivity. However, no immunoreactivity was detected in two areas of the brain known to contain dense OT-binding sites by receptor autoradiography studies: the ventral hippocampus and the central nucleus of the amygdala. In the pituitary, both the anterior and posterior lobes were positive for OT receptor immunoreactivity, whereas the intermediate lobe was negative. These results demonstrate that the same receptor type is expressed in both peripheral OT target tissues and the brain, and also suggest the possibility that a different OT receptor subtype may be present in some areas of the brain.