Draft genome of Tanacetum cinerariifolium, the natural source of mosquito coil
Draft genome of Tanacetum cinerariifolium, the natural source of mosquito coil
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DOI：
10.1038/s41598-019-54815-6
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发表时间：
2019-12-03
影响因子：
4.6
通讯作者：
Nakayama, Koji
中科院分区：
文献类型：
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作者：
Yamashiro, Takanori;Shiraishi, Akira;Nakayama, Koji
除虫菊（Tanacetum cinerariifolium）是一种多年生菊科植物，具有白色雏菊状的花，是蚊香的原始来源，并且以生物合成除虫菊酯类天然杀虫剂而闻名。然而，T.还没有完全阐明。在这里，我们提出了7.1-Gb的基因组草图T。cinerariifolium，由2，016，451个支架和60，080个高置信度预测的基因组成。值得注意的是，转座因子（TE）的分析表明，TE占基因组序列的33.84%。此外，在T.其中，瓜籽属特有的进化谱系占基因组序列的13%，比其他植物高出约8倍。InterProScan分析表明，生物防御相关的毒性蛋白（例如，核糖体失活蛋白），信号转导相关蛋白（例如，组氨酸激酶），和代谢酶（例如，脂氧合酶、酰基-CoA脱氢酶/加氧酶和P450）也在T.瓜籽基因组分子系统发育分析检测到多种酶与属特异性乘法，包括常见的酶和其他似乎是具体的除虫菊酯生物合成。总之，这些数据确定了除虫菊酯生物合成途径中可能的新组分，并为T.瓜籽
Pyrethrum (Tanacetum cinerariifolium), which is a perennial Asteraceae plant with white daisy-like flowers, is the original source of mosquito coils and is known for the biosynthesis of the pyrethrin class of natural insecticides. However, the molecular basis of the production of pyrethrins by T. cinerariifolium has yet to be fully elucidated. Here, we present the 7.1-Gb draft genome of T. cinerariifolium, consisting of 2,016,451 scaffolds and 60,080 genes predicted with high confidence. Notably, analyses of transposable elements (TEs) indicated that TEs occupy 33.84% of the genome sequence. Furthermore, TEs of the sire and oryco clades were found to be enriched in the T. cinerariifolium-specific evolutionary lineage, occupying a total of 13% of the genome sequence, a proportion approximately 8-fold higher than that in other plants. InterProScan analysis demonstrated that biodefense-related toxic proteins (e.g., ribosome inactivating proteins), signal transduction-related proteins (e.g., histidine kinases), and metabolic enzymes (e.g., lipoxygenases, acyl-CoA dehydrogenases/oxygenases, and P450s) are also highly enriched in the T. cinerariifolium genome. Molecular phylogenetic analysis detected a variety of enzymes with genus-specific multiplication, including both common enzymes and others that appear to be specific to pyrethrin biosynthesis. Together, these data identify possible novel components of the pyrethrin biosynthesis pathway and provide new insights into the unique genomic features of T. cinerariifolium.