MAST-CELLS OF 2 TYPES DIFFERING IN NEUTRAL PROTEASE COMPOSITION IN THE HUMAN AORTIC INTIMA - DEMONSTRATION OF TRYPTASE-CONTAINING AND TRYPTASE/CHYMASE-CONTAINING MAST-CELLS IN NORMAL INTIMAS, FATTY STREAKS AND THE SHOULDER REGION OF ATHEROMAS
MAST-CELLS OF 2 TYPES DIFFERING IN NEUTRAL PROTEASE COMPOSITION IN THE HUMAN AORTIC INTIMA - DEMONSTRATION OF TRYPTASE-CONTAINING AND TRYPTASE/CHYMASE-CONTAINING MAST-CELLS IN NORMAL INTIMAS, FATTY STREAKS AND THE SHOULDER REGION OF ATHEROMAS
复制标题
DOI：
10.1161/01.atv.14.6.966
复制
发表时间：
1994-06-01
期刊：
影响因子：
--
通讯作者：
KOVANEN, PT
中科院分区：
文献类型：
--
作者：
KAARTINEN, M;PENTTILA, A;KOVANEN, PT
体外生化研究表明，受刺激的肥大细胞通过肥大细胞颗粒、中性蛋白酶和蛋白多糖的协同作用诱导巨噬细胞泡沫细胞的形成。为了确定动脉内膜中肥大细胞的存在和数量，对35例13~67岁尸检的正常人和动脉粥样硬化者的主动脉内膜标本进行了类胰蛋白酶和乳糜酶两种主要的肥大细胞酶的单克隆抗体染色。所有存在的肥大细胞都含有类胰蛋白酶，平均40%的肥大细胞也含有凝乳酶。在正常内膜、脂肪条纹和动脉粥样硬化切片中，肥大细胞的平均密度分别为15/mm(2)、15/mm(2)和3/mm(2)。然而，与正常内膜和脂肪条纹相反，动脉粥样硬化的肥大细胞呈典型的不均匀分布：肩部肥大细胞8/mm(2)，纤维帽肥大细胞1/mm(2)，核心区没有肥大细胞。在正常内膜、脂肪条纹和动脉粥样硬化的肩部，肥大细胞占所有有核细胞的3%。正常内膜肥大细胞与T淋巴细胞和巨噬细胞的比例分别为2：1和1：4，脂肪条纹分别为1：3和1：10，肩部肥大细胞与T淋巴细胞和巨噬细胞的比例分别为1：5和1：20。因此，在人的主动脉内膜的血源性细胞中，肥大细胞构成了一个重要的细胞群，并且就其蛋白酶含量而言，这些内膜肥大细胞是异质性的。肥大细胞在动脉粥样硬化的脂肪条纹(泡沫细胞形成的部位)和肩部(即生长边缘)中的存在支持肥大细胞在动脉粥样硬化形成的早期和晚期都起作用的假说。
Biochemical studies in vitro have demonstrated that stimulated mast cells induce macrophage foam cell formation through the synergistic action of mast cell granule neutral proteases and proteoglycans. To determine the presence and number of mast cells in human arterial intima, the site of atherogenesis, specimens of normal and atherosclerotic human aortic intima from 35 autopsies of persons ranging from 13 to 67 years old were stained with monoclonal antibodies against the two major proteases of mast cells, tryptase and chymase. All mast cells present were found to contain tryptase, and an average of 40% contained chymase as well. In sections of normal intimas, fatty streaks, and atheromas, the mast cells had average densities of 15/mm(2), 15/mm(2), and 3/mm(2), respectively. In contrast to the normal intimas and fatty streaks, however, the atheromas had mast cells distributed unevenly in a typical pattern: 8/mm(2) in the shoulder region, 1/mm(2) in the fibrous cap, and none in the core region. In normal intimas, fatty streaks, and the shoulder region of atheromas, the mast cells amounted to 3% of all nucleated cells. The ratios of mast cells to T lymphocytes and to macrophages, respectively, were 2:1 and 1:4 in normal intimas, 1:3 and 1:10 in fatty streaks, and 1:5 and 1:20 in the shoulder region of atheromas. Thus, among the blood-borne cells in the human aortic intima, mast cells compose a significant cell population, and in terms of their protease content, these intimal mast cells are heterogeneous. The presence of mast cells in fatty streaks (the site of foam cell formation) and in the shoulder region, ie, the growing edge, of atheromas supports the hypothesis that mast cells play a role in both the early and late stages of atherogenesis.