Expression Profile and Estrogenic Regulation of Anti-Mullerian Hormone During Gonadal Development in Pejerrey Odontesthes bonariensis, a Teleost Fish With Strong Temperature-Dependent Sex Determination
Expression Profile and Estrogenic Regulation of Anti-Mullerian Hormone During Gonadal Development in Pejerrey Odontesthes bonariensis, a Teleost Fish With Strong Temperature-Dependent Sex Determination
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DOI：
10.1002/dvdy.21731
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发表时间：
2008-11-01
影响因子：
2.5
通讯作者：
Manuel Somoza, Gustavo
中科院分区：
文献类型：
--
作者：
Ignacio Fernandino, Juan;Shohei Hattori, Ricardo;Manuel Somoza, Gustavo
Pejerrey是一种硬骨鱼，表现出强烈的温度依赖性性别决定。本研究克隆了pejerrey amh基因的cDNA，分析了其在性腺分化过程中的表达谱，并与性腺芳香化酶（cyp 19 a1）的表达进行了比较。在性腺分化期，AMH在雄性化温度下的表达高于雌性化温度。在中等温度下（雌虫：雄虫1：1），半数幼虫表达量高，另一半表达量低。cyp 19 a1表现出相互的表达模式，AMH无论是单独和温度明智的。cyp 19 a1和amh的表达在性腺形态分化前就已增加。通过给予雌二醇而雌性化或通过给予芳香酶抑制剂而雄性化的幼虫中AMH表达分别下调和上调。这些结果表明，AMH在睾丸分化中起着关键作用，它显然是由雌激素在这个物种的调制。发展动力学237.3192-3199，2008年。(C)2008 Wiley-Liss，Inc.
Pejerrey is a teleost fish presenting a strong temperature-dependent sex determination. This study was conducted to clone pejerrey amh cDNA, analyze its expression profile during thermal and endocrine manipulation of gonadal differentiation, and compare its expression with that of gonadal aromatase (cyp19a1). Amh displayed higher expression at masculinizing than at feminizing temperatures during the gonadal differentiation period. Its expression at an intermediate temperature (females 1:1 males), was high in half of the larvae and low in the other half. Cyp19a1 showed a reciprocal expression pattern to that of amh both individually- and temperature-wise. Increased cyp19a1 and amh expression was observed before morphological gonadal differentiation. Amh expression in larvae feminized by administration of estradiol or masculinized by the administration of an aromatase inhibitor was down- and up-regulated, respectively. These results show that amh plays a critical role in testicular differentiation and it is apparently modulated by estrogens in this species. Developmental Dynamics 237.3192-3199, 2008. (C) 2008 Wiley-Liss, Inc.