The fractionation of nuclei from mammalian cells by zonal centrifugation.
The fractionation of nuclei from mammalian cells by zonal centrifugation.
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通过区带离心分离哺乳动物细胞的细胞核。
DOI：
10.1042/bj1090127
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发表时间：
1968
期刊：
影响因子：
--
通讯作者：
D. Ridge
中科院分区：
文献类型：
--
作者：
I. Johnston;A. Mathias;F. Pennington;D. Ridge
1.成年大鼠的纯化肝细胞核在低速区带转子中离心时分为两个主要区域。一个区域含有二倍体细胞核，另一个区域含有四倍体细胞核。2.研究了年龄对大鼠肝倍体模式的影响。四倍体细胞核在幼年动物中几乎不存在。随着年龄的增长，他们的比例稳步上升。部分肝切除扰乱了倍性模式。3.区带离心机可以从小鼠肝脏中分离出二倍体、四倍体、八倍体和十六倍体细胞核。4.大鼠肝细胞核与密度为1.35的蔗糖溶液在5 ℃下是等密度的。
1. Purified liver nuclei from adult rats separate into two main zones when centrifuged in the slow-speed zonal rotor. One zone contains diploid nuclei, the other tetraploid. 2. The effect of age on the pattern of rat liver ploidy was examined. Tetraploid nuclei are virtually absent from young animals. They increase in proportion steadily with age. Partial hepatectomy disturbs the pattern of ploidy. 3. The zonal centrifuge permits the separation of diploid, tetraploid, octaploid and hexadecaploid nuclei from mouse liver. 4. Rat liver nuclei are isopycnic with sucrose solutions of density 1.35 at 5 degrees .