METABOTROPIC GLUTAMATE RECEPTORS TRIGGER POSTSYNAPTIC PROTEIN-SYNTHESIS
METABOTROPIC GLUTAMATE RECEPTORS TRIGGER POSTSYNAPTIC PROTEIN-SYNTHESIS
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DOI：
10.1073/pnas.90.15.7168
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发表时间：
1993-08-01
影响因子：
11.1
通讯作者：
GREENOUGH, WT
中科院分区：
文献类型：
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作者：
WEILER, IJ;GREENOUGH, WT
K+去极化或谷氨酸添加到突触核小体制剂中，通过1 M蔗糖离心裂解液分离得到的多核糖体聚集体的大小会迅速增加。对谷氨酸类似物的反应谱与代谢受体的反应谱一致，这些类似物包括半胱甘酸酯、碘酸酯和1-氨基环戊烷-1,3-二羧酸酯。谷氨酸刺激由二酰基甘油类似物1-油基-2-乙酰甘油和蛋白激酶C激活剂二丁酸佛波醇模拟。磷脂酶阻滞剂2-硝基-4-羧基苯基-n、n -二苯基氨基甲酸酯和醌可减轻晚期反应。蛋白激酶C抑制剂calphostin C抑制对1-氨基环戊烷-1,3-二羧酸盐的反应。这些数据表明，谷氨酸能突触通过与磷脂酰肌醇第二信使系统偶联的代谢性谷氨酸受体上调突触后蛋白合成。这一机制可能是代谢性谷氨酸受体参与长期增强和其他形式的神经可塑性的基础。
K+ depolarization or addition of glutamate to a synaptoneurosome preparation triggers a rapid increase in size of polyribosomal aggregates isolated by centrifugation of lysate through 1 M sucrose. The profile of response to the glutamate analogues quisqualate, ibotenate, and 1-aminocyclopentane-1,3-dicarboxylate corresponds to that of metabotropic receptors. Glutamate stimulation is mimicked by the diacylglycerol analogue 1-oleoyl-2-acetylglycerol and by the protein kinase C activator phorbol dibutyrate. The phospholipase blockers 2-nitro-4-carboxyphenyl-N,N-diphenylcarbamate and quinacrine reduce the late phase of the response. The protein kinase C inhibitor calphostin C suppresses the response to 1-aminocyclopentane-1,3-dicarboxylate. These data indicate that glutamatergic synapses upregulate postsynaptic protein synthesis via metabotropic glutamate receptors coupled to the phosphatidylinositol second-messenger system. This mechanism could underlie the reported involvement of metabotropic glutamate receptors in long-term potentiation and other forms of neural plasticity.