No strict requirement for eosinophils for bone marrow plasma cell survival
No strict requirement for eosinophils for bone marrow plasma cell survival
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DOI：
10.1002/eji.201747229
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发表时间：
2018-05-01
影响因子：
5.4
通讯作者：
Allman, David
中科院分区：
文献类型：
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作者：
Bortnick, Alexandra;Chernova, Irene;Allman, David
持久的抗体应答由长寿的浆细胞维持，这些浆细胞被认为在BM中专门的生存小生境中。据报道，嗜酸性粒细胞作为BM生存生态位的关键组成部分发挥作用，它们被认为向附近的浆细胞提供促生存信号。最近的研究表明，许多BM浆细胞是最近产生的，主要是短寿命细胞，提高了罕见的浆细胞-嗜酸性粒细胞相互作用是建立持久的体液免疫所需的限速步骤的可能性。为了解决这些问题，我们研究了T细胞依赖性和T细胞非依赖性抗原诱导的抗体应答背景下嗜酸性粒细胞耗竭对短寿命和长寿命BM浆细胞的影响。令人惊讶的是，我们的研究结果未能支持嗜酸性粒细胞在浆细胞生成或存活中的作用。这些研究包括在抗体介导的耗竭后或由于GATA 1基因座突变而缺乏嗜酸性粒细胞的小鼠中检查浆细胞频率。
Lasting antibody responses are maintained by long-lived plasma cells, which are thought to lodge in the BM in specialized survival niches. Eosinophils have been reported to function as a critical component of the BM survival niche where they are thought to provide pro-survival signals to nearby plasma cells. Recent study shows that many BM plasma cells are recently generated and chiefly short-lived cells, raising the possibility that rare plasma cell-eosinophil interactions are a rate-limiting step needed to establish lasting humoral immunity. To address these issues, we examined the impact of eosinophil depletion on short-and long-lived BM plasma cells in the context of antibody responses induced by both T-cell dependent and T-cell independent antigens. Surprisingly, our results failed to support a role for eosinophils in either plasma cell generation or survival. These studies included examination of plasma cell frequencies in mice lacking eosinophils either after antibody-mediated depletion, or due to mutation of the GATA1 locus.