N-Cadherin Inhibits Schwann Cell Migration on Astrocytes
N-Cadherin Inhibits Schwann Cell Migration on Astrocytes
复制标题
N-钙粘蛋白抑制星形胶质细胞上的雪旺细胞迁移
DOI：
10.1006/mcne.1999.0766
复制
发表时间：
1999
影响因子：
3.5
通讯作者：
J. Fawcett
中科院分区：
文献类型：
--
作者：
M. Wilby;E. Muir;J. Fok;Barbara J. Gour;O. Blaschuk;J. Fawcett
星形胶质细胞在外周神经进入区将雪旺细胞（SC）从中枢神经系统（CNS）中排除，并限制其移植到CNS后的迁移。我们在体外模拟了SC、星形胶质细胞和成纤维细胞之间的相互作用。星形胶质细胞和SC在体外形成独立的领域，它们之间有明显的边界。当放置在星形胶质细胞单层上时，SC迁移较差，但在各种其他表面如层粘连蛋白（LN）和皮肤成纤维细胞上迁移良好。单个SC和星形胶质细胞之间的相互作用导致持久的粘附接触，在此期间SC不能从星形胶质细胞中迁移出来。相比之下，SC与成纤维细胞的相互作用要短得多，迁移停滞较少。SC强烈地粘附于星形胶质细胞和其他SC，但不太好地粘附于促进迁移的基质，如LN和成纤维细胞。SC-星形胶质细胞和SC-SC粘附由钙依赖性细胞粘附分子N-钙粘蛋白介导。通过钙撤退抑制N-钙黏着蛋白功能，含有经典钙黏着蛋白细胞粘附识别序列His-Ala-瓦尔的肽或针对该序列的抗体抑制SC粘附并增加SC在星形胶质细胞上的迁移。我们认为，N-钙粘蛋白介导的粘附星形胶质细胞抑制广泛迁移的SC在中枢神经系统组织。
Astrocytes exclude Schwann cells (SCs) from the central nervous system (CNS) at peripheral nerve entry zones and restrict their migration after transplantation into the CNS. We have modeled the interactions between SCs, astrocytes, and fibroblasts in vitro. Astrocytes and SCs in vitro form separate territories, with sharp boundaries between them. SCs migrate poorly when placed on astrocyte monolayers, but migrate well on various other surfaces such as laminin (LN) and skin fibroblasts. Interactions between individual SCs and astrocytes result in long-lasting adhesive contacts during which the SC is unable to migrate away from the astrocyte. In contrast, SC interactions with fibroblasts are much shorter with less arrest of migration. SCs adhere strongly to astrocytes and other SCs, but less well to substrates that promote migration, such as LN and fibroblasts. SC-astrocyte and SC-SC adhesion is mediated by the calcium-dependent cell adhesion molecule N-cadherin. Inhibition of N-cadherin function by calcium withdrawal, peptides containing the classical cadherin cell adhesion recognition sequence His-Ala-Val, or antibodies directed against this sequence inhibit SC adhesion and increase SC migration on astrocytes. We suggest that N-cadherin-mediated adhesion to astrocytes inhibits the widespread migration of SCs in CNS tissue.
N-钙粘蛋白在培养的少突胶质细胞中的表达和功能。
DOI：
10.1016/s0165-3806(96)00124-1
发表时间：
1996
期刊：
Brain research. Developmental brain research
影响因子：
--
作者：
Payne,HR;Hemperly,JJ;Lemmon,V
通讯作者：
Lemmon,V
轴突阻止雪旺细胞前体的迁移。
DOI：
10.1242/dev.120.6.1411
发表时间：
1994
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Bhattacharyya,A;Brackenbury,R;Ratner,N
通讯作者：
Ratner,N
影响因子：
6
作者：
Makrigiannakis, A;Coukos, G;Coutifaris, C
通讯作者：
Coutifaris, C