Estimation of Copper and Iron Burden in Biological Samples of Various Stages of Hepatitis C and Liver Cirrhosis Patients
Estimation of Copper and Iron Burden in Biological Samples of Various Stages of Hepatitis C and Liver Cirrhosis Patients
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DOI：
10.1007/s12011-014-0058-9
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发表时间：
2014-08-01
影响因子：
3.9
通讯作者：
Panhwar, Abdul Haleem
中科院分区：
文献类型：
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作者：
Arain, Salma Aslam;Kazi, Tasneem G.;Panhwar, Abdul Haleem
越来越多的证据表明，人体铁（Fe）和铜（Cu）的代谢因感染而发生改变，表明这两种元素在病毒性疾病的发病机制和进展中发挥作用。在本研究中，评估了不同阶段的男女丙型肝炎（丙型肝炎病毒（HCV））患者的生物样本（血清和头皮头发）中铜和铁的相关性。为了进行比较研究，收集了相同年龄组（30-50岁）和社会经济状况的健康个体的头皮毛发和血清样本。微波辅助酸消解后，通过原子吸收光谱分析生物样品中的铁和铜。采用相同基质的有证标准物质检验方法的有效性和准确性。与健康受试者相比，肝病患者的生物样本中铜和铁的水平升高，包括急性（诊断测试后，抗 HCV 血清阳性）肝纤维化和肝硬化。肝硬化患者的差异显着（p < 0.01）。据观察，两性参考者和患者的 Cu 和 Fe 数据均呈正态分布。除 HCV 第一阶段 (p < 0.1) 外，参考者和患者血清和头皮毛发样本中 Cu 与 Fe 的元素间相关性 (r) 在两性中均不显着 (p > 0.1)。结论是，人体内铜和铁含量的增加似乎有助于病毒性丙型肝炎患者发生肝硬化。
There is accumulative evidence that the metabolism of iron (Fe) and copper (Cu) is altered in human due to infections, indicating that both elements have roles in pathogenesis and progress of viral diseases. In the present study, the correlation of Cu and Fe was evaluate in biological samples (serum and scalp hair) of hepatitis C (hepatitis C virus (HCV)) patients of both genders at different stages. For comparative study, the scalp hair and serum samples of healthy individuals of same age group (30-50 years) and socioeconomic status were collected. The biological samples were analyzed for Fe and Cu by atomic absorption spectroscopy after microwave-assisted acid digestion. The validity and accuracy of methodology were checked by certified reference materials of same matrixes. The levels of Cu and Fe in biological samples were enhanced in hepatic disorder patients, including acute (after diagnosis test, anti-HCV sero-positive) hepatic fibrosis and liver cirrhosis as compared to healthy referents. The difference was significant (p < 0.01) in the case of liver cirrhotic patients. It was observed that the data of Cu and Fe in referents and patients of both genders had normal distributions. The inter-elemental correlation (r) among Cu vs Fe in serum and scalp hair samples of referents and patients were not significant in both genders (p > 0.1) except in the first stage of HCV (p < 0.1). It was concluded that the increase of Cu and Fe content in human body seems to contribute to the development of cirrhosis in patients with viral hepatitis C.