Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein
Identification and characterization of D-AKAP1 as a major adipocyte PKA and PP1 binding protein
复制标题
DOI：
10.1016/j.bbrc.2006.05.138
复制
发表时间：
2006-07-21
影响因子：
3.1
通讯作者：
Moorhead, Greg B. G.
中科院分区：
文献类型：
--
作者：
Bridges, Dave;MacDonald, Justin A.;Moorhead, Greg B. G.
蛋白激酶A （PKA）在调节脂肪细胞脂质代谢中起重要作用。已知PKA的活性受其在细胞中的特定位置的调节，这是一个由a激酶锚定蛋白（AKAPs）介导的过程。为了研究PKA在该组织中的亚细胞定位，我们在脂肪细胞中搜索了AKAP蛋白。我们纯化了一个120 kDa的蛋白，它可以结合PKA的调控亚基和蛋白磷酸酶1 （PP1）的催化亚基。该蛋白富集于原代脂肪细胞的脂滴部分，鉴定为D-AKAP1。该蛋白可能在脂肪细胞PKA的调控中起重要作用。(c) 2006爱思唯尔公司版权所有。
Protein kinase A (PKA) plays an important role in the regulation of lipid metabolism in adipocytes. The activity of PKA is known to be modulated by its specific location in the cell, a process mediated by A-kinase anchoring proteins (AKAPs). In order to examine the subcellular localization of PKA in this tissue we performed a search for AKAP proteins in adipocytes. We purified a 120 kDa protein which can bind both the regulatory subunit of PKA as well as the catalytic subunit of protein phosphatase 1 (PP1). This protein was found to be enriched in the lipid droplet fraction of primary adipocytes and was identified as D-AKAP1. This protein may play an important role in the regulation of PKA in adipocytes. (c) 2006 Elsevier Inc. All rights reserved.