Immunocytochemical evidence for the specific localization of aldose reductase in rat Sertoli cells.
Immunocytochemical evidence for the specific localization of aldose reductase in rat Sertoli cells.
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大鼠支持细胞中醛糖还原酶特异性定位的免疫细胞化学证据。
DOI：
10.1095/biolreprod26.2.311
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发表时间：
1982
影响因子：
3.6
通讯作者：
Hamilton,DW
中科院分区：
文献类型：
--
作者：
Ludvigson,MA;Waites,GM;Hamilton,DW
通过已建立的免疫细胞化学技术和多种方法，提出了醛糖还原酶(AR)在支持细胞中特异定位的证据。组织切片染色显示，这种酶在出生时就存在于支持细胞中，并且免疫细胞化学染色的强度在5日龄时比在老年大鼠的睾丸中更强。通过成熟睾丸的酶分散、未成熟大鼠睾丸中丰富的支持细胞制剂的培养以及在睾丸网液中收集新释放的精子，结果表明，除支持细胞外，其他类型的睾丸细胞中均不存在AR免疫反应。
Evidence that the enzyme aldose reductase(AR) is specifically located in Sertoli cells is presented by means of an established immunocytochemical technique and with a variety of approaches. By staining tissue sections, the enzyme was shown to be present in Sertoli cells at birth and the intensity of the immunocytochemical stain increased by 5 days of age to that found in the testes of older rats. By means of enzyme dispersion of mature testes; the culture of enriched Sertoli cell preparations from the testes of immature rats; and the collection of newly released testicular spermatozoa in rete testis fluid, it was shown that immunoreactive AR was not present in any testicular cell type except the Sertoli cell.