Genetic analyses of BaMMV/BaYMV resistance in barley accession HOR4224 result in the identification of an allele of the translation initiation factor 4e (Hv-eIF4E) exclusively effective against Barley mild mosaic virus (BaMMV).
Genetic analyses of BaMMV/BaYMV resistance in barley accession HOR4224 result in the identification of an allele of the translation initiation factor 4e (Hv-eIF4E) exclusively effective against Barley mild mosaic virus (BaMMV).
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对大麦种质 HOR4224 中 BaMMV/BaYMV 抗性的遗传分析结果鉴定出对大麦轻度花叶病毒 (BaMMV) 专门有效的翻译起始因子 4e (Hv-eIF4E) 等位基因。
DOI：
10.1007/s00122-014-2279-x
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发表时间：
2014
期刊：
影响因子：
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通讯作者：
Perovic D
中科院分区：
文献类型：
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作者：
Perovic D
关键信息基于将广泛的分离分析和等位性测试与等位基因特异性重测序相结合的策略，鉴定了仅对 BaMMV 有效的 Hv-eIF4E 等位基因，并开发了 BaYMV 抗性的紧密连锁标记。 摘要土传大麦黄花叶病是冬大麦最重要的病害之一。在广泛的抗性筛选中，鉴定出对三种大麦轻度花叶病毒株（BaMMV-ASL1、BaMMV-Sil和BaMMV-Teik）和两种大麦黄花叶病毒株（BaYMV-1和BaYMV-2）具有抗性的登记号“HOR4224”。使用 Bmac29 进行的分析在某种程度上可以诊断 therym4/5 位点，暗示该位点存在易感性编码等位基因。因此，筛选了源自‘HOR4224’בHOR10714’（易感）杂交的 107 个 DH 系的抗性。遗传分析揭示了对 BaMMV 和 BaYMV (= 5.58) 抗性的独立遗传，两者均由单个基因编码 (BaMMV= 0.477；BaYMV= 0.770)。虽然 Bmac29 表明易感性编码等位基因，但“HOR4224”的 BaMMV 抗性与rym4/rym5 共定位。BaYMV 抗性被映射到 rym3 区域中的染色体 5H。 Hv-eIF4E基因的全长cDNA的测序显示出已测序的等位基因，该等位基因被描述为对BaMMV和BaYMV有效。然而，‘HOR4224’和rym4/rym5供体杂交的F1后代均对BaMMV有抗性，但对BaYMV敏感。因此，这是第一个关于 therym4/rym5 位点上的等位基因专门针对 BaMMV 有效的报告。特异性的变化是由于一个非同义氨基酸取代 (I118K) 造成的。获得的结果阐明，将涉及 BaMMV/BaYMV 不同毒株的广泛分离分析和等位性测试与等位基因特异性重测序相结合，是复杂病理系统中基因和等位基因检测的有效策略。
Key messageBased on a strategy combining extensive segregation analyses and tests for allelism with allele-specific re-sequencing an Hv-eIF4E allele exclusively effective against BaMMV was identified and closely linked markers for BaYMV resistance were developed.AbstractSoil-borne barley yellow mosaic disease is one of the most important diseases of winter barley. In extensive screenings for resistance, accession ‘HOR4224’ being resistant to three strains ofBarley mild mosaic virus(BaMMV-ASL1, BaMMV-Sil, and BaMMV-Teik) and two strains ofBarley yellow mosaic virus(BaYMV-1 and BaYMV-2) was identified. Analyses using Bmac29, being to some extent diagnostic for therym4/5locus, gave hint to the presence of the susceptibility-encoding allele at this locus. Therefore, 107 DH lines derived from the cross ‘HOR4224’ × ‘HOR10714’ (susceptible) were screened for resistance. Genetic analyses revealed an independent inheritance of resistance to BaMMV and BaYMV (= 5.58) both encoded by a single gene (BaMMV= 0.477; BaYMV= 0.770). Although Bmac29 indicated the susceptibility-encoding allele, BaMMV resistance of ‘HOR4224’ co-localized withrym4/rym5.The BaYMV resistance was mapped to chromosome 5H in the region ofrym3. Sequencing of full length cDNA of theHv-eIF4Egene displayed an already sequenced allele described to be efficient against BaMMV and BaYMV. However, the F1progenies of crosses involving ‘HOR4224’ andrym4/rym5donors were all resistant to BaMMV but susceptible to BaYMV. Therefore, this is the first report of an allele at therym4/rym5locus exclusively efficient against BaMMV. Changes in the specificity are due to one non-synonymous amino acid substitution (I118K). Results obtained elucidate that combining extensive segregation analyses and tests for allelism involving different strains of BaMMV/BaYMV in combination with allele-specific re-sequencing is an efficient strategy for gene and allele detection in complex pathosystems.