Identification and localization of connexin26 within the photoreceptor-horizontal cell synaptic complex
Identification and localization of connexin26 within the photoreceptor-horizontal cell synaptic complex
复制标题
光感受器-水平细胞突触复合体中连接蛋白26的识别和定位
作者：
U. Janssen;K. Schultz;Alexandra Gellhaus;Peter Manuel Schmidt;J. Ammermüller;Reto Weiler
连接蛋白26（Cx 26）是一个完整的膜蛋白家族的成员，通常形成细胞间隙连接通道。我们采用Western blotting、免疫荧光、免疫电镜和单细胞逆转录聚合酶链反应扩增（RT-PCR）等方法分析了Cx 26在鲤鱼视网膜中的表达和细胞定位。在外丛状层中，Cx 26免疫标记集中在水平细胞的终末树突。单细胞RT-PCR证实了Cx 26在鲤鱼视网膜水平细胞中的表达。克隆了从四种不同水平细胞的cDNA扩增的248-bp片段，并且每个核苷酸序列编码与大鼠和小鼠Cx 26具有高度显著同源性的蛋白质片段（AA 104-185）。免疫电镜显示，只有水平细胞的内陷树突与突触前带状复合体密切的横向关联被标记。在感光细胞膜上没有发现标记，并且在感光细胞和水平细胞之间没有指示间隙连接的隔膜结构。Cx 26在水平细胞树突尖端的膜上的焦点位置和缺乏间隙连接形态表明Cx 26可能形成半通道。
Connexin26 (Cx26) is a member of the family of integral membrane proteins that normally form intercellular gap junctional channels. We have used Western blotting, immunofluorescence, immunoelectron microscopy, and single-cell reverse-transcriptase polymerase chain reaction amplification (RT-PCR) to analyze the expression and cellular localization of Cx26 in the carp retina. In the outer plexiform layer, strong clustered Cx26 immunolabeling was concentrated at and restricted to the terminal dendrites of horizontal cells. Single-cell RT-PCR confirmed the expression of Cx26 in carp retinal horizontal cells. 248-bp fragments amplified from cDNAs of four different horizontal cells were cloned and each nucleotide sequence encodes a protein fragment (AA 104-185) with highly significant homology to rat and mouse Cx26. Immunoelectron microscopy revealed that only the invaginating dendrites of horizontal cells in intimate lateral association with the presynaptic ribbon complex were labeled. No labeling was found at the photoreceptor membrane and there was no septalaminar structure, indicative of gap junctions, between photoreceptors and horizontal cells. The focal location of Cx26 at the membrane of the dendritic tips of horizontal cells and the lack of gap junctional morphology suggests that Cx26 might form hemichannels.
DOI：
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发表时间：
1996-07
期刊：
Cell growth & differentiation : the molecular biology journal of the American Association for Cancer Research
影响因子：
--
作者：
K. Hirschi;Cheng Xu;T. Tsukamoto;R. Sager
通讯作者：
K. Hirschi;Cheng Xu;T. Tsukamoto;R. Sager
影响因子：
2.5
作者：
DONG, CJ;MCREYNOLDS, JS
通讯作者：
MCREYNOLDS, JS
金鱼的水平细胞连接。
DOI：
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发表时间：
1982
期刊：
Progress in clinical and biological research
影响因子：
--
作者：
Stell,WK;Kretz,R;Lightfoot,DO
通讯作者：
Lightfoot,DO