IDENTIFICATION OF ACTIN-FILAMENTS IN THE RHABDOMERAL MICROVILLI OF DROSOPHILA PHOTORECEPTORS
IDENTIFICATION OF ACTIN-FILAMENTS IN THE RHABDOMERAL MICROVILLI OF DROSOPHILA PHOTORECEPTORS
复制标题
DOI：
10.1083/jcb.110.6.1993
复制
发表时间：
1990-06-01
影响因子：
7.8
通讯作者：
WILLIAMS, DS
中科院分区：
文献类型：
--
作者：
ARIKAWA, K;HICKS, JL;WILLIAMS, DS
节肢动物感光器的光传导微绒毛各自包含轴向细胞骨架。目前的研究表明，肌动蛋白丝是果蝇细胞骨架的组成部分。首先，通过用抗肌动蛋白进行免疫金标记，在横纹小球微绒毛和横纹小球下细胞质中检测到肌动蛋白。其次，用鬼笔环肽标记横纹肌球，表明丝状肌动蛋白的存在。最后，用肌球蛋白亚片段 1 装饰肌动蛋白丝。由 subfragment-1 修饰形成的特征性箭头复合体指向微绒毛的基部，因此每根细丝的快速生长端位于微绒毛的远端，在那里它被嵌入耐洗涤剂的帽中。每个微绒毛含有多于一根肌动蛋白丝。装饰的细丝延伸每个微绒毛的整个长度并投射到弹球下细胞质中。这种组织与肠刷状缘微绒毛中的肌动蛋白丝的组织相当。对小龙虾（原螯虾）的光感受器微绒毛也进行了类似的观察。我们的结果表明与横纹肌相关的任何肌球蛋白如何发挥作用。
The phototransductive microvilli of arthropod photoreceptors each contain an axial cytoskeleton. The present study shows that actin filaments are a component of this cytoskeleton in Drososphila. Firstly, actin was detected in the rhabdomeral microvilli and in the subrhabdomeral cytoplasm by immunogold labeling with antiactin. Secondly, the rhabdomeres were labeled with phalloidin, indicating the presence of filamentous actin. Finally, the actin filaments were decorated with myosin subfragment-1. The characteristic arrowhead complex formed by subfragment-1 decoration points towards the base of the microvilli, so that the fast growing end of each filament is at the distal end of the microvillus, where it is embedded in a detergent-resistant cap. Each microvillus contains more than one actin filament. Decorated filaments extend the entire length of each microvillus and project into the subrhabdomeral cytoplasm. This organization is comparable to that of the actin filaments in intestinal brush border microvilli. Similar observations were made with the photoreceptor microvilli of the crayfish, Procambarus. Our results provide an indication as to how any myosin that is associated with the rhabdomeres might function.