Comparative studies of Cu-64-ATSM and C-11-acetate in an acute myocardial infarction model:: Ex vivo imaging of hypoxia in rats
Comparative studies of Cu-64-ATSM and C-11-acetate in an acute myocardial infarction model:: Ex vivo imaging of hypoxia in rats
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DOI：
10.1016/s0969-8051(98)00049-3
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发表时间：
1999-01-01
影响因子：
3.1
通讯作者：
Welch, MJ
中科院分区：
文献类型：
--
作者：
Fujibayashi, Y;Cutler, CS;Welch, MJ
铜标记二乙酰基-双（N-4-甲基氨基硫脲）（Cu-ATSM）是一种很有前途的缺氧组织显像剂。在本研究中，使用Cu-64（t（1/2）~ 12.8 h）标记的Cu-ATSM与C-11（t（1/2）= 20.3 min）标记的乙酸盐组合作为局部灌注标记物，使用离体组织切片成像技术在急性左前降支（LAD）冠状动脉闭塞大鼠模型中显示缺氧大鼠心脏组织。静脉内注射Cu-64-ATSM。10 min后处死大鼠。在处死前1分钟注射碳-11-乙酸盐以获得血流测量值。将心脏解剖，冷冻，用计量切片机切成1 mm厚的切片，用电子放射自显影仪获得C-11图像。在C-11衰变后，以相同的方式获得Cu-64的图像。在缺血区域，有低C-11积累，Cu-64显示高积累时，与正常区域相比。在大面积闭塞的大鼠中，缺血中心没有显示任何C-11或Cu-64的积累，表明没有血液供应。Cu-ATSM似乎是有用的检测缺氧与对比被观察到在短时间内（10分钟）注射后。核医学生物学26;1：117-121 1999. (C)1998年爱思唯尔科学公司
Copper labeled diacetyl-bis(N-4-methylthiosemicarbazone) (Cu-ATSM) is a promising agent for the imaging of hypoxic tissues. In the present study Cu-64 (t(1/2) - 12.8 h) labeled Cu-ATSM was used in combination with C-11 (t(1/2) = 20.3 min) labeled acetate as a regional perfusion marker to visualize hypoxic rat heart tissue in an acute left anterior descending (LAD) coronary artery occluded rat model using an ex vivo tissue slice imaging technique. Cu-64-ATSM was injected intravenously c.a. 10 min after occlusion and rats were sacrificed by cervical dislocation 10 min after injection. Carbon-11-acetate was injected 1 min before sacrifice to obtain a measure of blood flow. The heart-was dissected, frozen, and cut into l-mm thick slices with a gauged slicer, and C-11 images were obtained with an electronic autoradiography instrument. After decay of C-11, Cu-64 images were obtained in the same manner. In ischemic regions, where there was low C-11 accumulation, Cu-64 showed high accumulation when compared with normal regions. In rats with a large occlusion, the center of the ischemia did not show any accumulation of either C-11 or Cu-64, indicating no blood supply. Cu-ATSM appears to be useful for the detection of hypoxia with contrast being observed at short times (10 min) postinjection. NUCL MED BIOL 26;1:117-121 1999. (C) 1998 Elsevier Science Inc.