Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu
Extraordinary expansion of a Sorangium cellulosum genome from an alkaline milieu
复制标题
碱性环境中纤维素基因组的非凡扩展
DOI：
10.1038/srep02101
复制
发表时间：
2013-07-01
影响因子：
4.6
通讯作者：
Li, Yue-zhong
中科院分区：
文献类型：
--
作者：
Han, Kui;Li, Zhi-feng;Li, Yue-zhong
复杂的环境条件可以通过未知的机制显着影响细菌基因组的大小。纤维堆囊菌So 0157 -2菌株是一种碱性适应性埃博霉素生产菌，在很宽的pH范围内生长。在这里，我们发现该菌株的基因组为14，782，125个碱基对，比S.赛璐珞此前报道。So 0157 - 2的11,599个编码序列（CDS）包含大量重复和水平转移的基因，受大量蛋白激酶、sigma因子和相关转录调控辅因子的调控，为So 0157 -2菌株提供了丰富的资源和生态适应的灵活性。比较转录组学的方法，其中检测到90.7%的总CDS，不仅表现出复杂的表达模式在不同的环境条件下，但也提出了一个碱改进的插入和复制的途径，这已被遗传学证明，在该菌株。这些结果为环境条件如何影响细菌基因组扩增提供了见解和范例。
Complex environmental conditions can significantly affect bacterial genome size by unknown mechanisms. The So0157-2 strain ofSorangium cellulosumis an alkaline-adaptive epothilone producer that grows across a wide pH range. Here, we show that the genome of this strain is 14,782,125 base pairs, 1.75-megabases larger than the largest bacterial genome fromS. cellulosumreported previously. The total 11,599 coding sequences (CDSs) include massive duplications and horizontally transferred genes, regulated by lots of protein kinases, sigma factors and related transcriptional regulation co-factors, providing the So0157-2 strain abundant resources and flexibility for ecological adaptation. The comparative transcriptomics approach, which detected 90.7% of the total CDSs, not only demonstrates complex expression patterns under varying environmental conditions but also suggests an alkaline-improved pathway of the insertion and duplication, which has been genetically testified, in this strain. These results provide insights into and a paradigm for how environmental conditions can affect bacterial genome expansion.