TRANSCRIPTIONAL MAPPING OF HUMAN RESPIRATORY SYNCYTIAL VIRUS
TRANSCRIPTIONAL MAPPING OF HUMAN RESPIRATORY SYNCYTIAL VIRUS
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DOI：
10.1128/jvi.52.2.364-369.1984
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发表时间：
1984-01-01
影响因子：
5.4
通讯作者：
WERTZ, GW
中科院分区：
文献类型：
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作者：
DICKENS, LE;COLLINS, PL;WERTZ, GW
人呼吸道合胞病毒的转录图谱是通过测定紫外线照射对病毒基因失活的动力学来确定的。将呼吸道合胞病毒感染的HEp-2细胞的单层细胞培养物暴露于UV光，并通过凝胶电泳和与10种已知病毒基因的克隆互补cDNA拷贝的斑点印迹杂交来监测残留病毒RNA的合成。相对于细胞内病毒基因组复制的UV敏感性计算10个单独病毒基因的靶大小。靶大小分析表明，10个病毒基因被转录为一个单一的转录单位，单个基因的转录依赖于所有先前的基因的转录。基因转录的顺序（根据编码的蛋白质命名）确定为从启动子开始如下：14 K，11 K，N，P，M，9.5K，36 K，F，24 K，L。
A transcriptional map for human respiratory syncytial virus was determined by measuring the kinetics of viral gene inactivation in response to UV irradiation. Monolayer cell cultures of respiratory syncytial virus-infected HEp-2 cells were exposed to UV light, and residual viral RNA synthesis was monitored by gel electrophoresis and by hybridization to dot blots of cloned complementary cDNA copies of the 10 known viral genes. Target sizes for the 10 individual viral genes were calculated relative to the UV sensitivity of intracellular viral genome replication. Target size analysis indicated that the 10 viral genes were transcribed as a single transcriptional unit and that the transcription of an individual gene was dependent on the prior transcription of all the preceding genes. The order of gene transcription (with nomenclature according to encoded proteins) was determined to proceed from the promoter as follows: 14K, 11K, N, P, M, 9.5K, 36K, F, 24K, L.