Lymphocyte locomotion and attachment on two-dimensional surfaces and in three-dimensional matrices.
Lymphocyte locomotion and attachment on two-dimensional surfaces and in three-dimensional matrices.
复制标题
DOI：
10.1083/jcb.92.3.747
复制
发表时间：
1982-03
期刊：
影响因子：
--
通讯作者：
Wilkinson PC
中科院分区：
文献类型：
--
作者：
Haston WS;Shields JM;Wilkinson PC
比较了小鼠外周淋巴结淋巴细胞在二维蛋白包被基质和三维基质上的粘附和运动。淋巴细胞没有坚持，或迁移，2-D基质吸血清或纤连蛋白包被的玻璃。它们确实附着在水合的三维胶原晶格上并在其中迁移。当胶原蛋白脱水形成二维表面时，淋巴细胞对其的附着减少。我们建议，淋巴细胞，这是粘附性差，能够附着和迁移在3-D矩阵的非粘附机制，如伪足通过矩阵中的间隙，这可以提供购买运动的离散分子间粘附的情况下的延伸和扩展。这一点得到了使用血清包被滤纸的研究的支持，因为淋巴细胞附着并迁移到孔径足够大（3或8 μ m）的滤纸上，以允许伪足渗透，但不附着到由相同材料（纤维素酯）制成但孔径较窄（0.22或0.45 μ m）的滤纸上。胶原凝胶中淋巴细胞运动的电影摄影研究也与上述假设一致，因为淋巴细胞显示出比通常在平面表面上看到的更多变的形态，形成许多小伪足，扩大到细胞和伪足之间产生明显的收缩。当淋巴细胞离开或经过这些延伸物时，这些延伸物通常相对于环境保持固定。这表明伪足插入凝胶基质的间隙中，并作为运动的锚定点。
The adhesion and locomotion of mouse peripheral lymph node lymphocytes on 2-D protein- coated substrata and in 3-D matrices were compared. Lymphocytes did not adhere to, or migrate on, 2-D substrata suck as serum- or fibronectin-coated glass. They did attach to and migrate in hydrated 3-D collagen lattices. When the collagen was dehydrated to form a 2-D surface, lymphocyte attachment to it was reduced. We propose that lymphocytes, which are poorly adhesive, are able to attach to and migrate in 3-D matrices by a nonadhesive mechanism such as the extension and expansion of pseudopodia through gaps in the matrix, which could provide purchase for movement in the absence of discrete intermolecular adhesions. This was supported by studies using serum-coated micropore filters, since lymphocytes attached to and migrated into filters with pore sizes large enough (3 or 8 mum) to allow pseudopod penetration but did not attach to filters made of an identical material (cellulose esters) but of narrow pore size (0.22 or 0.45 mum). Cinematographic studies of lymphocyte locomotion in collagen gels were also consistent with the above hypothesis, since lymphocytes showed a more variable morphology than is typically seen on plane surfaces, with formation of many small pseudopodia expanded to give a marked constriction between the cell and the pseudopod. These extensions often remained fixed with respect to the environment as the lymphocyte moved away from or past them. This suggests that the pseudopodia were inserted into gaps in the gel matrix and acted as anchorage points for locomotion.