An ultraportable and versatile point-of-care DNA testing platform
An ultraportable and versatile point-of-care DNA testing platform
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DOI：
10.1126/sciadv.aaz7445
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发表时间：
2020-04
期刊：
影响因子：
13.6
通讯作者：
Huan Xu;A. Xia;Dandan Wang;Yiheng Zhang;S. Deng;Weiping Lu;Jie Luo;Qiu Zhong;Fengling Zhang;Lin Zhou;Wenqing Zhang;Yang Wang;Cheng Yang;Kai Chang;Weiling Fu;Jinhui Cui;Mingzhe Gan;D. Luo;Ming Chen
中科院分区：
文献类型：
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作者：
Huan Xu;A. Xia;Dandan Wang;Yiheng Zhang;S. Deng;Weiping Lu;Jie Luo;Qiu Zhong;Fengling Zhang;Lin Zhou;Wenqing Zhang;Yang Wang;Cheng Yang;Kai Chang;Weiling Fu;Jinhui Cui;Mingzhe Gan;D. Luo;Ming Chen
超便携式 POCT 平台，用于从诊所到农业、环境到食品应用的 DNA 检测。即时检测 (POCT) 在资源有限的环境中具有广泛的应用。这里，展示了一种称为 POCKET（整个测试的即时护理套件）的 POCT 平台，该平台具有超便携性和多功能性，可以以从样本到答案的方式分析不同领域的多种类型的 DNA。 POCKET重量小于100克，长度小于25厘米。该套件由集成芯片（i-chip）和可折叠盒子（f-box）组成。 i-chip 将样品制备与之前未知的三重信号放大功能集成在一起。 f-box 使用智能手机作为加热器、信号检测器和结果读数器。我们检测到了从诊所到环境、食品到农业的不同类型的 DNA。检测灵敏（保质期 10 周）。这种廉价、超便携的 POCKET 平台可能成为临床诊断、食品安全、农业保护和环境监测的多功能样本到答案平台。
An ultraportable POCT platform for DNA detection from clinics to agriculture to environment to food applications. Point-of-care testing (POCT) has broad applications in resource-limited settings. Here, a POCT platform termed POCKET (point-of-care kit for the entire test) is demonstrated that is ultraportable and versatile for analyzing multiple types of DNA in different fields in a sample-to-answer manner. The POCKET is less than 100 g and smaller than 25 cm in length. The kit consists of an integrated chip (i-chip) and a foldable box (f-box). The i-chip integrates the sample preparation with a previously unidentified, triple signal amplification. The f-box uses a smartphone as a heater, a signal detector, and a result readout. We detected different types of DNA from clinics to environment to food to agriculture. The detection is sensitive (10 weeks shelf life). This inexpensive, ultraportable POCKET platform may become a versatile sample-to-answer platform for clinical diagnostics, food safety, agricultural protection, and environmental monitoring.