Influence of light on growth, fumonisin biosynthesis and FUM1 gene expression by Fusarium proliferatum
Influence of light on growth, fumonisin biosynthesis and FUM1 gene expression by Fusarium proliferatum
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DOI：
10.1016/j.ijfoodmicro.2011.10.031
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发表时间：
2012-02-01
影响因子：
5.4
通讯作者：
Mule, Giuseppina
中科院分区：
文献类型：
--
作者：
Fanelli, Francesca;Schmidt-Heydt, Markus;Mule, Giuseppina
伏马菌素是一组真菌毒素，主要存在于玉米和以玉米为原料的食品和饲料中，与几种动物疾病有关。这些毒素对世界范围内的经济和健康的影响推动了一些努力，以澄清环境因素的作用，这些因素可能影响产毒素物种的伏马菌素生物合成。本文分析了不同波长的光对增殖镰刀菌（Fusarium proliferatum） item1719生长和伏马菌素合成的影响。总的来说，光对生长有积极的影响，与黑暗孵育相比，光照下的生长速度平均提高了约40%。与暗光孵育相比，来自光谱两侧的波长，从长（627 nm）到短波长（470- 455nm）对伏马菌素的生物合成有刺激作用：伏马菌素B-1 （FB1）和B-2 （FB2）的产量在红光下增加了约40倍，在蓝光下增加了35倍，在天蓝色下增加了20倍，在绿光下增加了10倍，在黄光下增加了5倍，在白光下增加了3倍。采用Real time逆转录酶PCR定量分析了富马菌素生物合成基因FUM1的转录调控，揭示了富马菌素生物合成与基因表达之间的相关性。这些发现显示了光对伏马菌素生长和生物合成的调节作用，并为一种重要的产毒玉米病原体的生理学提供了新的信息。(C) 2011 Elsevier B.V.版权所有
Fumonisins are a group of mycotoxins, mainly found in maize and maize-based food and feed, associated with several diseases in animals. The impact of these toxins on the economy and health worldwide has driven several efforts to clarify the role of environmental factors that can influence fumonisin biosynthesis by the toxigenic species. We analyzed the influence of light of varying wavelength on growth and fumonisin biosynthesis by the fungus Fusarium proliferatum ITEM 1719. Light in general had a positive influence on growth, with a mean increase of the grow rate of about 40% under light exposure in comparison to the dark incubation. Wavelengths from both sides of the spectrum, from long (627 nm) to short wavelength (470-455 nm) had a stimulating effect on fumonisin biosynthesis compared to the dark incubation: fumonisins B-1 (FB1) and B-2 (FB2) production increased of about 40 fold under red, 35 fold under blue, 20 fold under royal blue, 10 fold under green, 5 fold under yellow and 3 fold under white light in comparison to the dark incubation. The transcriptional regulation of the FUM1 fumonisin biosynthesis gene was analyzed by Real time reverse transcriptase PCR quantification, revealing a correlation between fumonisin biosynthesis and gene expression. These findings show a role of light on the growth and the modulation of fumonisin biosynthesis and provide new information on the physiology of an important toxigenic maize pathogen. (C) 2011 Elsevier B.V. All rights reserved.