Ganglioside Synthesis during the Development of Neuronal Polarity
Ganglioside Synthesis during the Development of Neuronal Polarity
复制标题
神经元极性发育过程中的神经节苷脂合成
DOI：
10.1074/jbc.271.25.14876
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发表时间：
1996
期刊：
影响因子：
--
通讯作者：
A. Futerman
中科院分区：
文献类型：
--
作者：
K. Hirschberg;R. Zisling;G. van Echten;A. Futerman
神经节苷脂的水平和类型在神经元分化和发育期间发生变化，但没有研究将这些变化与神经元形态发生中的特定事件联系起来。在这里，我们分析了神经节苷脂的合成和发展的轴突和树突的极化神经元之间的关系，使用海马神经元培养的方式，轴突和树突产生的一个定义的事件序列，其中几乎没有污染的胶质细胞。用[4，5 - 3 H]二氢鞘氨醇标记神经元，其迅速掺入细胞并代谢为3 H标记的鞘糖脂。3 H-标记的鞘糖脂合成速率与[4，5 - 3 H]二氢鞘氨醇摄取的初始速率成正比，并且在孵育9 h内与时间呈线性关系。3 H标记的神经节苷脂合成的主要变化发生在轴突发生和轴突快速生长期间。在轴突生成过程中，有一个显着增加的合成复杂的神经节苷脂（即GM 1，GD 1a，GD 1b和GT 1b）与相应的减少合成的葡萄糖神经酰胺和神经节苷脂GD 3。在轴突快速生长阶段，a-与b-系列神经节苷脂的比例显着增加。然而，在树突发生，树突生长和突触发生，神经节苷脂的合成几乎没有变化，与一个小的和逐渐增加的比例的a-到b-系列的神经节苷脂和增加的神经节苷脂GD 1a和GT 1b的合成。这些结果表明，尽管神经元的形态和功能的神经元成熟的重大变化，神经节苷脂合成的变化仅限于神经元发育的早期阶段，即轴突发生和快速轴突伸长。
Changes in the levels and types of gangliosides occur during neuronal differentiation and development, but no studies have correlated these changes with defined events in neuronal morphogenesis. Here, we have analyzed the relationship between ganglioside synthesis and the development of axons and dendrites in polarized neurons, using hippocampal neurons cultured in such a way that axons and dendrites are generated by a defined sequence of events and in which there is virtually no contamination by glial cells. Neurons were labeled with [4,5-3H]dihydrosphingosine, which was rapidly incorporated into cells and metabolized to 3H-labeled glycosphingolipids. The rate of 3H-labeled glycosphingolipid synthesis was directly proportional to the initial rate of [4,5-3H]dihydrosphingosine uptake and was linear versus time for up to 9 h of incubation. The major changes in 3H-labeled ganglioside synthesis occurred during the period of axonogenesis and rapid axon growth. During axonogenesis, there was a significant increase in the synthesis of complex gangliosides (i.e. GM1, GD1a, GD1b, and GT1b) with a corresponding reduction in the synthesis of glucosylceramide and ganglioside GD3. During the stage of rapid axon growth, the ratio of a- to b-series gangliosides increased significantly. However, during dendritogenesis, dendrite growth, and synaptogenesis, there was little change in ganglioside synthesis, with a small and gradual increase in the ratio of a- to b-series gangliosides and an increase in the synthesis of gangliosides GD1a and GT1b. These results indicate that despite major changes in neuronal morphology and functionality as neurons mature, changes in ganglioside synthesis are restricted to early stages of neuronal development, namely axonogenesis and rapid axon elongation.
神经节苷脂唾液酸化的发育模式与培养的胚胎鸡脑神经元的神经细胞发生一致。
DOI：
--
发表时间：
1992
期刊：
The Journal of biological chemistry
影响因子：
--
作者：
Rosenberg,A;Sauer,A;Noble,EP;Gross,HJ;Chang,R;Brossmer,R
通讯作者：
Brossmer,R