Cryopreservation of extracted corneal lenticules after small incision lenticule extraction for potential use in human subjects.
Cryopreservation of extracted corneal lenticules after small incision lenticule extraction for potential use in human subjects.
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DOI：
10.1097/ico.0000000000000276
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发表时间：
2014-12
期刊：
影响因子：
2.8
通讯作者：
Rao PA
中科院分区：
文献类型：
--
作者：
Ganesh S;Brar S;Rao PA
描述小切口屈光性角膜摘出术（ReLEx SMILE）后摘出的角膜小珠的冷冻保存技术和飞秒激光基质内晶状体植入术治疗远视的初步结果。从接受ReLEx SMILE矫正近视的患者中收集晶状体，并进行组织处理技术和冷冻保存。这些眼贴随后用于治疗8只远视眼和1只无晶状体眼。使用飞秒激光在每位患者的角膜中创建一个囊袋，然后植入冷冻保存的囊袋。通过随访检查对患者进行监测，平均155.4天（38-310天）。从微球储存到从液氮中取出的平均间隔为96天（范围，19-178天）。接受治疗的远视眼的平均等效球镜为+4.50 ± 1.1屈光度（D）。平均角膜曲率和角膜厚度分别从术前的43.9 D和531.6 μm变为术后的47.4 D和605.2 μm。远视眼的平均剩余等效球镜为+0.6 D，无晶状体眼为+4.1 D。在随访期结束时，没有一只眼睛显示出排斥或最佳矫正视力丧失的证据。冷冻保存技术似乎是一种长期储存ReLEx SMILE后提取的屈光性角膜的安全方法，可用于同种异体人类受试者。由于退变、混浊、角膜瓣相关并发症、术后干眼症和高阶像差的风险较低，它可能是准分子激光消融术治疗远视的安全有效的替代方案。临床试验注册-URL：http://www.clinicaltrials.gov。唯一标识符：CTRI/2014/01/004331。
To describe the technique of cryopreservation of corneal lenticules extracted after small incision refractive lenticule extraction (ReLEx SMILE) and initial results of femtosecond laser intrastromal lenticular implantation for hyperopia. Lenticules were collected from patients undergoing ReLEx SMILE for the correction of myopia and subjected to a tissue processing technique and cryopreservation. These lenticules were subsequently used to treat 8 hyperopic eyes and 1 aphakic eye. A femtosecond laser was used to create a pocket into each patient's cornea, followed by implantation of a cryopreserved lenticule. The patients were monitored through follow-up examinations for a mean 155.4 days (38–310 days). The mean interval from storage of lenticules to removal from liquid nitrogen was 96 days (range, 19–178 days). Mean spherical equivalent of hyperopic eyes treated was +4.50 ± 1.1 diopter (D). Mean keratometry and pachymetry changed from preoperative 43.9 D and 531.6 μm to 47.4 D and 605.2 μm, respectively, postoperatively. Mean residual spherical equivalent for hyperopic eyes was +0.6 D and +4.1 D for the aphakic eye. None of the eyes showed evidence of rejection or loss of best-corrected visual acuity at the end of the follow-up period. The cryopreservation technique seems to be a safe method of long-term storage of refractive lenticules extracted after ReLEx SMILE for use in allogeneic human subjects. It may potentially be a safe and effective alternative to excimer laser ablation for hyperopia because of the low risks of regression, haze, flap-related complications, postoperative dry eye, and higher-order aberrations. Clinical Trial Registration—URL: http://www.clinicaltrials.gov. Unique identifier: CTRI/2014/01/004331.