Muscarinic agonists and phorbol esters increase tyrosine phosphorylation of a 40-kilodalton protein in hippocampal slices.
Muscarinic agonists and phorbol esters increase tyrosine phosphorylation of a 40-kilodalton protein in hippocampal slices.
复制标题
毒蕈碱激动剂和佛波醇酯会增加海马切片中 40 千道尔顿蛋白质的酪氨酸磷酸化。
DOI：
10.1073/pnas.86.7.2498
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发表时间：
1989
影响因子：
11.1
通讯作者：
Baraban,JM
中科院分区：
文献类型：
--
作者：
Stratton,KR;Worley,PF;Huganir,RL;Baraban,JM
本实验采用海马脑片制备方法，研究了脑内蛋白酪氨酸磷酸化的调控。完整切片的药理学处理后，蛋白质通过电泳分离，蛋白质酪氨酸磷酸化水平通过免疫印迹与特异性抗磷酸酪氨酸抗体进行评估。佛波酯，丝氨酸和苏氨酸磷酸化酶蛋白激酶C的激活剂，选择性地增加具有约40千道尔顿的表观分子量的可溶性蛋白的酪氨酸磷酸化。强烈激活肌醇磷脂系统的毒蕈碱激动剂如卡巴胆碱和氧化震颤素M也增加该蛋白的酪氨酸磷酸化。肌醇磷脂系统和蛋白激酶C的神经递质激活似乎触发了导致酪氨酸磷酸化增加的级联反应。
We have used the hippocampal slice preparation to investigate the regulation of protein tyrosine phosphorylation in brain. After pharmacological treatment of intact slices, proteins were separated by electrophoresis, and levels of protein tyrosine phosphorylation were assessed by immunoblotting with specific anti-phosphotyrosine antibodies. Phorbol esters, activators of the serine- and threonine-phosphorylating enzyme protein kinase C, selectively increase tyrosine phosphorylation of a soluble protein with an apparent molecular mass of approximately 40 kilodaltons. Muscarinic agonists such as carbachol and oxotremorine M that strongly activate the inositol phospholipid system also increase tyrosine phosphorylation of this protein. Neurotransmitter activation of the inositol phospholipid system and protein kinase C appears to trigger a cascade leading to increased tyrosine phosphorylation.