Mouse cathepsin K: cDNA closing and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development
Mouse cathepsin K: cDNA closing and predominant expression of the gene in osteoclasts, and in some hypertrophying chondrocytes during mouse development
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DOI：
10.1016/0014-5793(96)00907-6
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发表时间：
1996-09-16
期刊：
影响因子：
3.5
通讯作者：
Vuorio, E
中科院分区：
文献类型：
--
作者：
Rantakokko, J;Aro, HT;Vuorio, E
We have constructed cDNA clones covering the entire coding region of mouse, human and rabbit preprocathepsin It mRNA for studies on bone turnover, The clone pMCatK-1 for mouse cathepsin K shares 87% nucleotide homology with the corresponding human and rabbit sequences, Analysis of a panel of mouse tissues for tissue distribution of cathepsin K mRNA revealed the highest levels in musculoskeletal tissues: bone, cartilage and skeletal muscle, In situ hybridization of developing mouse embryos was performed to identify the cellular source of cathepsin K mRNA, The strongest mRNA signal was detected in osteoclasts of bone, identified in serial sections by positive TRAP staining, Cathepsin K mRNA was also observed in some hypertrophic chondrocytes of growth cartilages. Association of cathepsin K production with degradation of bone and cartilage matrix suggests that this enzyme and its mRNA levels could serve as markers for matrix degradation in diseases affecting these tissues.
