Heterogeneity of vertebrate brain tubulins.
Heterogeneity of vertebrate brain tubulins.
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脊椎动物脑微管蛋白的异质性。
DOI：
10.1073/pnas.81.13.4041
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发表时间：
1984
影响因子：
11.1
通讯作者：
Lee,JC
中科院分区：
文献类型：
--
作者：
Field,DJ;Collins,RA;Lee,JC
我们使用等电聚焦、二维电泳和肽图谱程序检查了微管蛋白研究中常用的六种脊椎动物（大鼠、小牛、猪、鸡、人类和羔羊）的脑微管蛋白异质性程度，这些程序提供了比以前更高的分辨率。根据异微管蛋白的数量、等电点范围和分布来判断，所有这些生物体的异质性程度极其相似。从所有来源中至少分离出 6 个 α 和 12 个 β 微管蛋白。甚至二维肽图上的斑点图案也非常相似。这些相似之处表明所有这些大脑中的微管蛋白群体应该具有相似的整体物理特性。特别有趣的是，只有四五个β微管蛋白基因的鸡却含有大约12个β微管蛋白。因此，翻译后修饰必须至少产生一些微管蛋白异质性。含有 15-20 个微管蛋白 DNA 序列的哺乳动物物种并不比鸡表现出更多的微管蛋白异质性。这表明可能只需要少量哺乳动物基因的表达来产生观察到的微管蛋白异质性。
We have examined the extent of brain tubulin heterogeneity in six vertebrate species commonly used in tubulin research (rat, calf, pig, chicken, human, and lamb) using isoelectric focusing, two-dimensional electrophoresis, and peptide mapping procedures that provide higher resolution than previously available. The extent of heterogeneity is extremely similar in all of these organisms, as judged by number, range of isoelectric points, and distribution of the isotubulins. A minimum of 6 alpha and 12 beta tubulins was resolved from all sources. Even the pattern of spots on two-dimensional peptide maps is remarkably similar. These similarities suggest that the populations of tubulin in all of these brains should have similar overall physical properties. It is particularly interesting that chicken, which has only four or five beta-tubulin genes, contains approximately 12 beta tubulins. Thus, post-translational modification must generate at least some of the tubulin heterogeneity. Mammalian species, which contain 15-20 tubulin DNA sequences, do not show any more tubulin protein heterogeneity than does chicken. This suggests that expression of only a small number of the mammalian genes may be required to generate the observed tubulin heterogeneity.