Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle

Biological Properties of the Aggregated Form of Chitosan Magnetic Nanoparticle
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DOI：
10.21873/invivo.11966
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发表时间：
2020-07-01
期刊：
IN VIVO
影响因子：
2.3
通讯作者：
Garcia-Contreras, Rene
Garcia-Contreras, Rene
中科院分区：
医学4区
文献类型：
--
作者：
David Paulino-Gonzalez, Angel;Sakagami, Hiroshi;Garcia-Contreras, Rene

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背景/目的：壳聚糖包覆的氧化铁纳米颗粒（Chi-NP）因其生物相容性、生物可降解性、低毒性和磁场下的靶向性而受到关注。在这项研究中，我们研究了 Chi-NP 的各种生物学特性。材料和方法：ChiNP 通过将磁性 NP 与壳聚糖 FL-80 混合来制备。通过扫描和透射电子显微镜测定颗粒大小，通过MTT测定细胞活力，通过细胞分选仪测定细胞周期分布，通过组合指数测定与抗癌药物的协同作用，通过ELISA测定人牙龈成纤维细胞中PGE(2)的产生。结果：从 FL-80 和磁性 NP 合成 Chi-NP 的过程增加了与细胞的亲和力，达到纳米纤维达到的水平。与培养基接触后，Chi-NP 立即形成聚集体并干扰细胞内的摄取。聚集的 Chi-NP 没有表现出细胞毒性、与抗癌药物的协同作用、诱导细胞凋亡（亚 G1 细胞群的积累）、保护细胞免受 X 射线诱导的损伤，也不影响基础和 IL-1 β 诱导的 PGE 产生。结论：Chi-NP 具有生物惰性，并且与细胞表现出高亲和力，进一步证实了其作为药物递送支架的优越性。
Background/Aim: Chitosan-coated iron oxide nanoparticles (Chi-NP) have gained attention because of their biocompatibility, biodegradability, low toxicity and targetability under magnetic field. In this study, we investigated various biological properties of Chi-NP. Materials and Methods: ChiNP was prepared by mixing magnetic NP with chitosan FL-80. Particle size was determined by scanning and transmission electron microscopes, cell viability by MTT assay, cell cycle distribution by cell sorter, synergism with anticancer drugs by combination index, PGE(2) production in human gingival fibroblast was assayed by ELISA. Results: The synthetic process of Chi-NP from FL-80 and magnetic NP increased the affinity to cells, up to the level attained by nanofibers. Upon contact with the culture medium, Chi-NP instantly formed aggregates and interfered with intracellular uptake. Aggregated Chi-NP did not show cytotoxicity, synergism with anticancer drugs, induce apoptosis (accumulation of subG1 cell population), protect the cells from X-ray-induced damage, nor affected both basal and IL-1 beta-induced PGE, production. Conclusion: Chi-NP is biologically inert and shows high affinity to cells, further confirming its superiority as a scaffold for drug delivery.