Vacuoles in the Acrysof™ intraocular lens as factor of the presence of serum in aqueous humor
Vacuoles in the Acrysof™ intraocular lens as factor of the presence of serum in aqueous humor
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DOI：
10.1159/000055645
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发表时间：
2001-03-01
影响因子：
2.1
通讯作者：
Pfeiffer, N
中科院分区：
文献类型：
--
作者：
Dick, HB;Olson, RJ;Pfeiffer, N
目的：Acrysof（TM）（Alcon）可折叠晶状体（IOL）已被证明具有高度生物相容性，并且显示出较低的后囊膜混浊发生率。然而，在一些Acrysof人工晶状体中观察到微小的空泡或“闪光”。空泡形成的临床相关性目前尚不清楚。为了帮助阐明体内条件下存在的因素对空泡形成的影响，本实验研究检查了Acrysof IOL中房水成分对空泡发生的影响。研究方法：在体温（37 ℃）下，在有或无人血清的前房房水中培养总共12枚无菌Acrysof IOL（Alcon，MA60BM）3或6个月。从2枚未经处理、未经水合、无菌对照IOL和12枚经处理IOL上切下中心部分，并使用光学显微镜检查空泡形成。使用光学显微镜和扫描电子显微镜检查第三个未经调节的水合对照IOL。测量：空泡的发生率由两名独立的研究者使用光学显微镜进行量化。用平衡盐溶液水合后，使用扫描电子显微镜检查对照IOL的表面质量。结果：对照IOL没有空泡或空泡很少。扫描电子显微镜显示对照IOL具有正常的表面纹理，无任何表面空泡。在不含血清的房水中调节的IOL没有表现出空泡数量的大幅增加，而在含血清的房水中调节的IOL表现出随时间增加的更多空泡数量。结论：在含血清的房水中，空泡的数量随孵育时间的延长而增加。血清的加入增加了溶液中脂质和蛋白质的比例，这也发生在血-水屏障的破坏中。本研究的结果指出了可能导致IOL中空泡形成的生理因素，并可帮助临床医生识别与空泡形成有关的风险因素。版权所有（C）2001 S. Karger AG，巴塞尔。
Objective: Acrysof(TM) (Alcon) foldable lenses (IOLs) have been shown to be highly biocompatible and exhibit a low incidence of posterior capsular opacification. However, minute vacuoles or 'glistenings' have been observed in some Acrysof IOLs. The clinical relevance of vacuole formation is presently unclear. To help clarify the influence of factors present under in vivo conditions on vacuole formation, the present experimental study examines the influence of aqueous humor components on the occurrence of vacuoles in Acrysof IOLs. Methods: A total of 12 sterile Acrysof IOLs (Alcon, MA60BM) were incubated at body temperature (37 degreesC) for 3 or 6 months in anterior-chamber aqueous humor with or without human serum. The center portion was cut from two unconditioned, unhydrated, sterile control IOLs and the 12 conditioned IOLs and examined using light microscopy for vacuole formation. A third unconditioned, hydrated control IOL was examined using light and scanning electron microscopy. Measures: The incidence of vacuoles was quantifled by two independent investigators using light microscopy. After hydration with balanced salt solution, the surface quality of a control IOL was examined using scanning electron microscopy. Results: The control IOLs exhibited no or very few vacuoles. Scanning electron microscopy revealed that the control IOLs had normal surface texture without any surface vacuoles. IOLs that had been conditioned in aqueous humor without serum exhibited no great increase in the number of vacuoles, whereas IOLs conditioned in aqueous humor with serum exhibited a greater number of vacuoles that increased over time. Conclusions: The number of vacuoles increases with incubation time in aqueous humor containing serum. The addition of serum increased the proportion of lipids and proteins in the solution, which also occurs with a breakdown in the blood-aqueous barrier. The results of the present study point to a physiological factor that may lead to vacuole formation in IOLs and may aid clinicians in identifying risk factors involved in the formation of vacuoles. Copyright (C) 2001 S. Karger AG, Basel.