Induction of H2O2-metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit
Induction of H2O2-metabolizing enzymes and total protein synthesis by antagonistic yeast and salicylic acid in harvested sweet cherry fruit
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DOI：
10.1016/j.postharvbio.2005.10.009
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发表时间：
2006-03-01
影响因子：
7
通讯作者：
Tian, SP
中科院分区：
文献类型：
--
作者：
Chan, ZL;Tian, SP
将甜樱桃果实浸泡在浓度为5 × 107个细胞/ml的Pichia membranefaciens或0.5 mM的水杨酸（SA）中10 min可降低扩展青霉引起的腐烂发生率和病变大小。在未接种病原菌的情况下，酵母菌处理提高了果实过氧化物酶（POD）活性，但降低了过氧化氢酶（CAT）和超氧化物歧化酶（SOD）活性。SA处理显著抑制CAT活性，但促进SOD和POD活性。接种P. expansum后，酵母和SA处理的果实中CAT活性降低，SOD活性升高。各处理间POD活性与水分对照差异不显著。酵母和SA处理改变了POD同工酶的表达。此外，酵母和SA处理提高了甜樱桃的总蛋白含量，并上调了SDS-PAGE显示的33和47 kDa蛋白条带。这些结果表明，酵母和SA处理诱导抗氧化酶和特异性蛋白质的合成，这可能在抗采后蓝霉病中发挥作用。(c)2005 Elsevier B. V.保留所有权利。
The immersion of sweet cherry fruit in Pichia membranefaciens at a concentration of 5 x 107 cells ml(-1) or in salicyclic acid (SA) at 0.5 mM for 10 min reduced the incidence of decay and lesion size caused by Penicillium expansum. Without pathogen inoculation, peroxidase (POD) activity was enhanced in yeast-treated fruit, but activities of catalase (CAT) and superoxide dismutase (SOD) showed a decrease in the same fruit. SA-treatment significantly inhibited CAT activity, but stimulated SOD and POD activities. After inoculation with P. expansum, CAT activity decreased and SOD activity increased in both yeast- and SA-treated fruit. No obvious difference was found in POD activity between treatments and water control. Treatments with yeast and SA changed the expression of POD isozymes. In addition, yeast and SA treatment increased total protein content of sweet cherry and up-regulated 33 and 47 kDa protein bands shown by SDS-PAGE. These results indicated that yeast- and SA-treatments induced synthesis of anti-oxidant enzymes and specific proteins, which may play a role in the resistance against postharvest blue mold. (c) 2005 Elsevier B.V. All rights reserved.