Rapid and Simple Identification of Orientia tsutsugamushi from Other Group Rickettsiae by Duplex PCR Assay Using groEL Gene
Rapid and Simple Identification of Orientia tsutsugamushi from Other Group Rickettsiae by Duplex PCR Assay Using groEL Gene
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利用 groEL 基因进行双重 PCR 检测，快速、简单地鉴定恙虫病东方体和其他立克次体
DOI：
10.1111/j.1348-0421.2005.tb03760.x
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发表时间：
2005
影响因子：
2.6
通讯作者：
Seung
中科院分区：
文献类型：
--
作者：
Hyo‐Soon Park;Jung‐hee Lee;E. Jeong;Jung;Sung‐Jin Hong;Tae;Tae‐Yung Kim;W. Jang;Kyung‐hee Park;Bum;Y. Kook;Seung
本研究建立了两种新的基于groEL基因的双重PCR方法，并对该方法在立克次体诊断中的应用进行了研究。第一次双重PCR分别扩增了斑疹伤寒群（TG）和斑点热群（SFG）立克次体等6株立克次体和5株恙虫病群（STG）立克次体的229-bp和366-bp DNA。第二次双重PCR检测扩增了6株立克次体菌株和5株STG菌株的397-bp和213-bp DNA。该方法可在一次反应中同时进行立克次体的快速鉴定和STG与其它群立克次体的鉴别诊断。
In this study, two new duplex PCR methods based on the groEL gene were developed and investigated for the diagnosis of rickettsiae. The first duplex PCR assay amplified the 229‐bp and the 366‐bp DNAs of 6 strains including typhus group (TG) and spotted fever group (SFG) rickettsiae, and 5 scrub typhus group (STG) rickettsiae, respectively. The second duplex PCR assay amplified the 397‐bp and the 213‐bp DNAs of 6 Rickettsia strains and 5 STG strains. These duplex PCR methods could simultaneously perform the rapid identification of rickettsiae and the differential diagnosis of STG and other group rickettsiae in a single reaction.