A METHOD FOR USING MICROSPHERES TO MEASURE MUSCLE BLOOD-FLOW IN EXERCISING RATS
A METHOD FOR USING MICROSPHERES TO MEASURE MUSCLE BLOOD-FLOW IN EXERCISING RATS
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DOI：
10.1152/jappl.1982.52.6.1629
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发表时间：
1982-01-01
影响因子：
3.3
通讯作者：
ROUK, K
中科院分区：
文献类型：
--
作者：
LAUGHLIN, MH;ARMSTRONG, RB;ROUK, K
需要一种导管植入程序，允许使用放射性标记微球（MS）技术来测量大鼠在高速跑步机上的骨骼肌血流量（BF）。尝试使用现有程序是不成功的。硅胶导管(0.02英寸；ID[内径].times。0.037英寸。将OD（外径）放置在升主动脉（用于MS输注）和肾动脉（用于提取参考样本）中，最大限度地减少了这些运动表现问题。然后有必要确定主动脉MS输注导致良好的MS-血混合。实验方法如下：比较左室（LV）灌注后和主动脉灌注后主动脉2个部位的参考提取液的放射性；比较左室和主动脉灌注麻醉大鼠和清醒大鼠双侧配对组织的BF值；组织学研究MS在肌肉中的分布；比较主动脉MS灌注大鼠在跑步机上双侧配对组织的BF变化。在近端和远端参考提取样本中发现的放射性差异百分比显然与左室和主动脉MS输注相同。双侧配对组织样本的BF与左室或主动脉MS输注相同。肌内MS分布均匀，MS聚集无明显问题。运动大鼠对双侧配对组织样本的BF值相同。这项技术可以用来测量在跑步机上跑步的大鼠的肌肉BF。
A catheter-implantation procedure allowing use of the radiolabeled microsphere (MS) technique for measuring skeletal muscle blood flow (BF) in rats during high-speed treadmill running was desired. Attempts to use existing procedures were unsuccessful. Silastic catheters (0.02 in. ID [inner diameter] .times. 0.037 in. OD [outer diameter]) placed in the ascending aorta (for MS infusions) and the renal artery (for reference sample withdrawal) minimized these exercise performance problems. It was then necessary to establish that aortic MS infusions result in good MS-blood mixing. The method was tested with the following: the radioactivities found in reference withdrawal samples taken from 2 locations in the aorta were compared after left ventricular (LV) infusion and after aortic infusions; BF to bilaterally paired tissues were compared in anesthetized and conscious rats with LV and aortic infusions; the distribution of MS in the muscles was studied histologically; and BF in bilaterally paired tissues were compared in rats with aortic MS infusions during treadmill running. The percent difference between the radioactivities found in the proximal and distal reference withdrawal samples evidently was the same for LV and aortic MS infusions. BF to bilaterally paired tissue samples was the same with both LV or aortic MS infusions. The MS were distributed uniformly within muscles, and MS aggregation was not a significant problem. BF to bilaterally paired tissue samples were the same in exercising rats. This technique can be used to measure muscle BF in rats running on a treadmill.