Sweetness reception in man: the multipoint attachment theory
Sweetness reception in man: the multipoint attachment theory
复制标题
男人的甜蜜接受：多点依恋理论
DOI：
10.1016/0308-8146(96)00023-4
复制
发表时间：
1996
期刊：
影响因子：
8.8
通讯作者：
J. Tinti
中科院分区：
文献类型：
--
作者：
C. Nofre;J. Tinti
多点附着（MPA）理论假设人类甜味受体（可能是七次跨膜受体）中至少存在八个基本识别位点，即 B-、AH-、XH-、G1-、G2-、G3-、G4- 和 D-识别位点。 B-识别位点假定为 Lys 残基； AH 和 XH 识别位点、Asp（或 Glu）残基； G1、G2、G3 和 G4 识别位点、Thr 残基；和D-识别位点，Ser（或Thr）残基。这八个识别位点能够与八个甜味剂相互作用位点相互作用，即B、AH、XH、G1、G2、G3、G4和D相互作用位点。给定甜味剂中相互作用位点的数量可以等于或小于八个。除 D 外，交互位点由两个基本交互位点（交互点或子位点）组成。这些相互作用点（即B1、B2、AH1、AH2、XH1、XH2、G1、E1、G2、E2、G3、E3、G4、E4和D点）通过三种类型的基本相互作用与受体相互作用，即离子相互作用、氢键相互作用和空间相互作用。 B(B1、B2)为阴离子基团(CO2-)或H键受体原子(Cl、Br、O)； AH1、AH2、XH1、XH2 是氢键供体基团（NH+、NH、OH）； E1、E2、E3、E4为H键受体原子(N、O、Cl、Br)； G1、G2、G3、G4 是空间相互作用亚位（CH3、CH2、CH、卤素）； D是H键受体基团(CN)。给出了几个例子来说明该理论。例如，根据MPA理论，D-葡萄糖是B1、B2、AH1、AH2、XH1、XH2型甜味剂，D-果糖是E1、E2、E3、E4型甜味剂，蔗糖是B1、B2、AH1、AH2、XH1、XH2、G1、E1、G2、E2、G3、E3、G4、 E4型甜味剂。
The multipoint attachment (MPA) theory assumes the presence, in the human sweetness receptor (probably a seven-pass transmembrane receptor), of at least eight fundamental recognition sites, namely, the B-, AH-, XH-, G1-, G2-, G3-, G4- and D-recognition sites. The B-recognition site is assumed to be a Lys residue; the AH- and XH-recognition sites, Asp (or Glu) residues; the G1-, G2-, G3- and G4-recognition sites, Thr residues; and the D-recognition site, a Ser (or Thr) residue. These eight recognition sites are able to interact with eight sweetener interaction sites, namely, the B, AH, XH, G1, G2, G3, G4 and D interaction sites. The number of interaction sites in a given sweetener may be equal to or lower than eight. Apart from D, the interaction sites are made up of two elementary interaction sites (interaction points or subsites). These interaction points (namely, the B1, B2, AH1, AH2, XH1, XH2, G1, E1, G2, E2, G3, E3, G4, E4 and D points) operate with the receptor through three types of elementary interactions, namely, ionic, H-bonding and steric interactions. B (B1, B2) is an anionic group (CO2−) or an H-bond acceptor atom (Cl, Br, O); AH1, AH2, XH1, XH2 are H-bond donor groups (NH+, NH, OH); E1, E2, E3, E4 are H-bond acceptor atoms (N, O, Cl, Br); G1, G2, G3, G4 are steric interaction subsites (CH3, CH2, CH, halogens); and D is an H-bond acceptor group (CN). Several examples are given to illustrate the theory. For example, according to the MPA theory, D-glucose is a B1, B2, AH1, AH2, XH1, XH2-type sweetener, D-fructose an E1, E2, E3, E4-type sweetener, and sucrose a B1, B2, AH1, AH2, XH1, XH2, G1, E1, G2, E2, G3, E3, G4, E4-type sweetener.