The catalog of human hair keratins I. Expression of the nine type I members in the hair follicle
The catalog of human hair keratins I. Expression of the nine type I members in the hair follicle
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DOI：
10.1074/jbc.274.28.19874
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发表时间：
1999-07-09
影响因子：
4.8
通讯作者：
Schweizer, J
中科院分区：
文献类型：
--
作者：
Langbein, L;Rogers, MA;Schweizer, J
人I型毛发角蛋白亚家族包含9个单独的成员，可细分为三组。A组（hHa1、hHa3 - I、hHa3 - II、hHa4）和B组（hHa7、hHa8）各自包含结构相关的毛发角蛋白，而C组的成员hHa2、hHa5和hHa6代表结构上相关性较弱的毛发角蛋白。针对这些单独的毛发角蛋白产生的抗体，首先通过毛发角蛋白总量的一维蛋白质印迹分析其特异性，然后用于建立人I型毛发角蛋白亚家族的二维图谱。该图谱包含两个不同系列的I型毛发角蛋白：一个是强表达、可被考马斯亮蓝染色的系列，包含毛发角蛋白hHa1、hHa3 - I/II、hHa4和hHa5；另一个是弱表达、可通过免疫检测的系列，包含hHa2、hHa6、hHa7和hHa8。对头皮毛囊的原位杂交和免疫组织化学表达研究表明，两种毛发角蛋白hHa2和hHa5界定了毛发分化的早期阶段，即hHa5在毛母质中表达，hHa5/hHa2在早期毛小皮细胞中共表达。虽然毛小皮分化过程中没有其他I型毛发角蛋白的表达，但毛母质细胞通过依次表达hHa1、hHa3 - I/II和hHa4进入皮质分化途径，在皮质分化的后期阶段还有hHa6作为补充，并且hHa8在皮质细胞中异质性表达。因此，6种I型毛发角蛋白参与了生长期毛发的终末分化。hHa7的表达与其他毛发角蛋白明显不同，它不出现在终末头皮毛发的大型生长期毛囊中，而仅出现在产生毳毛的罕见且小型毛囊类型的中央皮质细胞中。
The human type I hair keratin subfamily comprises nine individual members, which can be subdivided into three groups. Group A (hHa1, hHa3-I, hHa3-II, hHa4) and B (hHa7, hHa8) each contains structurally related hair keratins, whereas group C members hHa2, hHa5, and hHa6 represent structurally rather unrelated hair keratins. Antibodies produced against these individual hair keratins, first analyzed for specificity by one- dimensional Western blots of total hair keratins, were used to establish the two-dimensional catalog of the human type I hair keratin subfamily. The catalog comprises two different series of type I hair keratins: a strongly expressed, Coomassie-stainable series containing hair keratins hHa1, hHa3-I/II, hHa4, and hHa5, and a weakly expressed, immunodetectable series harboring hHa2, hHa6 hHa7, and hHa8. In situ hybridization and immunohistochemical expression studies on scalp follicles show that two hair keratins, hHa2 and hHa5, define the early stage of hair differentiation, i.e. hHa5 expression in hair matrix and hHa5/hHa2 coexpression in the early hair cuticle cells. Whereas cuticular differentiation proceeds without the expression of further type I hair keratins, matrix cells embark on the cortical pathway by sequentially expressing hHa1, hHa3-I/II, and hHa4, which are supplemented by hHa6 at an advanced stage of cortical differentiation, and hHa8, which is expressed heterogeneously in cortex cells. Thus, six type I hair keratins are involved in the terminal differentiation of anagen hairs. The expression of hHa7 is conspicuously different from that of the other hair keratins in that it does not occur in the large anagen follicles of terminal scalp hairs but only in central cortex cells of the rare and small follicle type that gives rise to vellus hairs.