Differences in biomarkers of cartilage matrix turnover and their changes over 2years in adolescent and adult volleyball athletes.
Differences in biomarkers of cartilage matrix turnover and their changes over 2years in adolescent and adult volleyball athletes.
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DOI：
10.1186/s40634-017-0081-9
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发表时间：
2017-12-01
影响因子：
1.8
通讯作者：
Duda, Georg N
中科院分区：
文献类型：
--
作者：
Boeth, Heide;MacMahon, Aoife;Duda, Georg N
背景技术背景：本研究旨在评估软骨转换的生物标志物的纵向变化的可行性，并确定其与患者评定的结果超过2年的排球运动员.METHODS：37名运动员进行了研究：18名青少年（年龄15.9 ± 0.64岁），在2年的密集排球训练计划和19名成人休闲排球运动员（年龄46.5 ± 4.9岁）。在基线（BL）和2年随访（FU）时采集血液和血清样本。受试者完成了国际膝关节文献委员会（IKDC）的主观膝关节形式和简短的形式36（SF-36）在BL。结果：13名青少年（72%）有开放的生长板在BL（BL开放的青少年），其余有封闭的生长板在BL（BL封闭的青少年），和所有，但一个青少年有封闭的生长板在FU的MRI评估。与成人相比，BL开放和闭合青少年具有更高水平的基于软骨降解的生物标志物II型胶原蛋白45聚体胶原酶肽（C2C-HUSA）和II型胶原蛋白C端肽（CTX-II）。BL开放青少年显示C2CHUSA、胶原合成标记物II型前胶原C-前肽（CPII）和CTXII减少，成人显示软骨中间层蛋白2（CILP-2）和C2C-HUSA增加。在青少年中，IKDC评分与CPII变化相关。在成年人中，SF-36身体成分评分与软骨寡聚基质蛋白（COMP）的变化相关。结论：生物标志物水平随时间的显着差异显示评估其变化的可行性。青少年中C2C-HUSA和CTX-II水平高于成人可能反映了关节负荷增加引起的软骨周转增加。CPII和COMP可能更能反映患者的主观结局。因此，这些生物标志物可能有助于评估机械负荷引起的软骨变化，其相关症状和运动员骨关节炎风险。
BACKGROUND: This study aimed the feasibility to assess longitudinal changes in biomarkers of cartilage turnover and to determine their relationship with patient-rated outcomes over 2 years in volleyball athletes.METHODS: Thirty-seven athletes were studied: 18 adolescents (age 15.9 ± 0.64 years) in a 2-year intensive volleyball training program and 19 adult recreational volleyball players (age 46.5 ± 4.9 years). Blood and serum samples were taken at baseline (BL) and 2-year follow-up (FU). Subjects completed the International Knee Documentation Committee (IKDC) Subjective Knee Form and the Short-Form 36 (SF-36) at BL.RESULTS: Thirteen adolescents (72%) had open growth plates at BL (BL open adolescents), the rest had closed growth plates at BL (BL closed adolescents), and all but one adolescent had closed growth plates at FU as assessed by MRI. BL open and closed adolescents had greater levels of the cartilage degradation-based biomarkers 45 mer collagenase peptide of type II collagen (C2C-HUSA) and C-telopeptide of type II collagen (CTX-II) than adults. BL open adolescents showed decreases in C2CHUSA, collagen synthesis marker C-propeptide of type II procollagen (CPII), and CTXII, and adults showed increases in cartilage intermediate layer protein 2 (CILP-2) and C2C-HUSA. In adolescents, IKDC scores were correlated with CPII changes. In adults, SF-36 Physical Component Scores were correlated with cartilage oligomeric matrix protein (COMP) changes.CONCLUSION: Significant differences in biomarker levels over time show the feasibility to assess their changes. Greater levels of C2C-HUSA and CTX-II in adolescents than in adults may reflect increased cartilage turnover in response to higher joint loading. CPII and COMP may be more reflective of subjective patient outcomes. These biomarkers may thus be useful in assessing mechanical loading-induced cartilage changes, their associated symptoms, and Osteoarthritis risk in athletes.