RNA PROCESSING FACTOR3 Is Crucial for the Accumulation of Mature ccmC Transcripts in Mitochondria of Arabidopsis Accession Columbia
RNA PROCESSING FACTOR3 Is Crucial for the Accumulation of Mature ccmC Transcripts in Mitochondria of Arabidopsis Accession Columbia
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DOI：
10.1104/pp.111.181552
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发表时间：
2011-11-01
期刊：
影响因子：
7.4
通讯作者：
Binder, Stefan
中科院分区：
文献类型：
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作者：
Jonietz, Christian;Forner, Joachim;Binder, Stefan
RNA加工因子1（RPF 1）和RPF 2是参与拟南芥（Arabidopsis thaliana）中不同线粒体mRNA的5'加工的五肽重复（PPR）蛋白。这两个因子与育性恢复因子（RF）高度相似，RF是各种植物细胞质雄性不育/恢复系统的一部分。这些发现表明RF样PPR蛋白在转录后5'加工中的主导作用。为了进一步探索这组蛋白质的功能，我们研究了一些携带相应PPR基因插入的T-DNA系。这种筛选确定了一个几乎完全没有成熟的ccmC转录在At 1g 62930 T-DNA插入线，表型，可以恢复完整的At 1g 62930基因导入突变体。在这个核基因中的插入，我们现在称之为RPF 3，也导致线粒体中CcmC蛋白的严重减少。对缺少功能性RPF 3基因的C24/rpf 3 -1 F2杂种的分析表明，该基因对来自C24和其他种质的线粒体DNA中发现的独特的ccmC 5'上游构型的成熟ccmC 5'转录物末端的产生影响较小。这些数据表明，RF样蛋白的特定功能只需要与一个独特的mtDNA配置连接。我们的新研究结果进一步证实了类RF PPR蛋白在植物线粒体5'转录本末端转录后生成中的重要作用。
RNA PROCESSING FACTOR1 (RPF1) and RPF2 are pentatricopeptide repeat (PPR) proteins involved in 5' processing of different mitochondrial mRNAs in Arabidopsis (Arabidopsis thaliana). Both factors are highly similar to RESTORERS OF FERTILITY (RF), which are part of cytoplasmic male sterility/restoration systems in various plant species. These findings suggest a predominant role of RF-like PPR proteins in posttranscriptional 5' processing. To further explore the functions of this group of proteins, we examined a number of T-DNA lines carrying insertions in the corresponding PPR genes. This screening identified a nearly complete absence of mature ccmC transcripts in an At1g62930 T-DNA insertion line, a phenotype that could be restored by the introduction of the intact At1g62930 gene into the mutant. The insertion in this nuclear gene, which we now call RPF3, also leads to a severe reduction of the CcmC protein in mitochondria. The analysis of C24/rpf3-1 F2 hybrids lacking functional RPF3 genes revealed that this gene has less influence on the generation of the mature ccmC 5' transcript end derived from a distinct ccmC 5' upstream configuration found in mitochondrial DNAs from C24 and other accessions. These data show that a particular function of an RF-like protein is required only in connection with a distinct mtDNA configuration. Our new results further substantiate the fundamental role of RF-like PPR proteins in the posttranscriptional generation of plant mitochondrial 5' transcript termini.