Immunohistochemical distribution of inwardly rectifying K+ channels in the medulla oblongata of the rat
Immunohistochemical distribution of inwardly rectifying K+ channels in the medulla oblongata of the rat
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DOI：
10.1292/jvms.70.265
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发表时间：
2008-03-01
影响因子：
1.2
通讯作者：
Taniguchi, Kazuyuki
中科院分区：
文献类型：
--
作者：
Yamamoto, Yoshio;Ishikawa, Ryota;Taniguchi, Kazuyuki
内向整流K+通道Kir1.1、Kir2.3和Kirz4.1-Kir5.1是CO2/ H+的候选化学感受分子。我们确定了这些通道在延髓的mRNA表达和免疫组织化学定位。的老鼠。RT-PCR分析显示，在延髓中检测到Kir1.1、Kir2.3、Kir4.1和Kir5.1的mRNA。Kir1.1、Kir2.3、Kir 4的免疫反应性。1和Kir5.1在髓质中的表达，其免疫标记模式因亚基而异。Kir1.1和Kir2.3在化学感受区[孤束核（NTS）、中缝隐核（RO）、前Botzinger复合体（PreBotC）]和非化学感受区[舌下神经核（XII）、下橄榄核（10）]的神经细胞体和胶质细胞中观察到免疫反应性。Kir4.1在神经胶质细胞和神经胶质细胞中呈阳性反应，尤其在XII和IO中。Kir5.1免疫反应在XII、RO和PreBotC的神经细胞体中观察到，但在NTS或IO中未观察到。在NTS中，静脉曲张的神经纤维的密集网络显示Kir5.1的免疫反应性。我们的研究结果表明，Kir通道可能不采取具体的中央化学感受，但在各种神经元和神经胶质细胞的细胞膜的离子特性进行调节。
The inwardly rectifying K+ channels, Kir1.1, Kir2.3 and Kirz4.1-Kir5.1, are the candidate chemosensory molecules for CO2/ H+. We determined the mRNA expression and immunohistochemical localization of these channels in the medulla oblongata. of the rat. RT-PCR analysis revealed mRNAs of Kir1.1, Kir2.3, Kir4.1 and Kir5.1 were detected in the medulla. The immunoreactivities for Kir1.1, Kir2.3, Kir4. 1, and Kir5.1 were observed in the medulla, and immunolabeling pattern was varied by the subunit. Immunoreactivities for Kir1.1 and Kir2.3 were observed in the nerve cell bodies and glial cells both in the chemosensory areas [nucleus tractus solitarius (NTS), nucleus raphe obscurus (RO), pre-Botzinger complex (PreBotC)] and non-chemosensory area [hypoglossal nucleus (XII), inferior olive nucleus (10)]. Kir4.1 immunoreactivity was observed in the glial cells and neuropil, especially in XII and IO. Kir5.1 immunoreactivity was observed in the nerve cell bodies in the XII, RO, and PreBotC, but not in the NTS or IO. In the NTS, a dense network of varicose nerve fibers showed immunoreactivity for Kir5.1. Our findings suggest that Kir channels may not act specific to the central chemoreception, but regulate the ionic properties of cellular membranes in various neurons and glial cells.