The nirSTBM region coding for cytochrome cd 1‐dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono‐, di‐, and tetraheme proteins
The nirSTBM region coding for cytochrome cd
1‐dependent nitrite respiration of Pseudomonas stutzeri consists of a cluster of mono‐, di‐, and tetraheme proteins
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编码细胞色素 cd 的 nirSTBM 区域
DOI：
10.1016/0014-5793(91)80150-2
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发表时间：
1991
期刊：
影响因子：
3.5
通讯作者：
W. Zumft
中科院分区：
文献类型：
--
作者：
A. Jüngst;S. Wakabayashi;H. Matsubara;W. Zumft
斯氏假单胞菌的呼吸亚硝酸盐还原(反硝化)基因聚集在7kbp以内。对含有细胞色素1结构基因nirS的4.6kbpHindIII-KpnI片段进行了测序。NirScode下游的一个开放阅读框编码一个22.8 kDa的蛋白质，带有四个半乳糖结合基序。该基因的突变导致细胞色素1电子供体的明显缺陷。紧随其后的是细胞色素552、细胞色素551和ORF5的结构基因，ORF5编码11.9 kDa的单血红素蛋白。所有的细胞色素都有一个蛋白质输出的信号序列。
Genes for respiratory nitrite reduction (denitrification) ofPseudomonas stutzeriare clustered within 7 kbp. A 4.6‐kbpHindIII‐KpnI fragment carryingnirS, the structural gene for cytochromecd1, was sequenced. An open reading frame immediately downstream ofnirScodes for a 22.8‐kDa protein with four hemec‐binding motifs. Mutagenesis of this gene causes an apparent defect in electron donation to cytochromecd1. Following this ORF are the structural genes for cytochromec552, cytochromec551, and ORF5 that codes for a 11.9‐kDa monoheme protein. All cytochromes have a signal sequence for protein export.