Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium
Suppression of norovirus by natural phytochemicals from Aloe vera and Eriobotryae Folium
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DOI：
10.1016/j.foodcont.2016.10.051
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发表时间：
2017-03-01
期刊：
影响因子：
6
通讯作者：
Song, Moon Jung
中科院分区：
文献类型：
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作者：
Ng, Yee Ching;Kim, Ye Won;Song, Moon Jung
人类诺如病毒（HuNoV）是病毒性食源性疾病的主要原因，但目前尚无有效的药物。这项研究报道了芦荟 (AV) 和枇杷叶 (EF) 天然提取物的抗诺如病毒活性。鼠诺如病毒 1 (MNV-1)（HuNoV 的替代病毒）与 AV 或 EF 提取物预孵育，然后在 RAW 264.7 细胞中进行噬菌斑测定。 AV 和 EF 提取物的预处理有效抑制了 MNV-1 的感染性，估计半数抑制浓度 (IC50) 分别为 0.778 和 1.196 mg/mL。在 RAW 264.7 细胞中未观察到 AV 和 EF 的细胞毒性。 AV、芦荟素和芦荟大黄素中发现的蒽醌化合物显示出中等的抗病毒作用。当新鲜卷心菜表面接种 MNV-1 时，AV 和 EF 处理以及芦荟素和芦荟大黄素在 25 ℃ 下处理 30 分钟，可以像溶液中一样有效地抑制 MNV-1 在卷心菜表面的感染性，表明 AV 和 EF 的抗病毒活性可以应用于食品表面。这些结果表明，AV 和 EF 可能作为潜在有效且安全的诺如病毒抑制剂，因为它们的细胞毒性相对较低，这使得它们可以制备成食品安全制剂。 (C) 2016 Elsevier Ltd. 保留所有权利。
Human norovirus (HuNoV) is the major cause of viral foodborne diseases, but no effective medication is currently available. This study reported the anti-norovirus activity of the natural extracts of Aloe vera (AV) and Eriobotryae Folium (EF). Murine norovirus 1 (MNV-1), a surrogate virus of HuNoV, was preincubated with AV or EF extracts and then subjected to plaque assays in RAW 264.7 cells. Pretreatment of AV and EF extracts effectively suppressed the infectivity of MNV-1 with an estimated half-maximal inhibitory concentration (IC50) of 0.778 and 1.196 mg/mL, respectively. Cytotoxicity of AV and EF was not observed in RAW 264.7 cells. Anthraquinone compounds found in AV, aloin and aloe-emodin showed moderate antiviral effects. When fresh cabbage was inoculated with MNV-1 on the surface, AV and EF treatments as well as aloin and aloe-emodin at 25 degrees C for 30 min suppressed the MNV-1 infectivity on cabbage surface as effectively as in solution, suggesting the antiviral activity of AV and EF can be applied on food surface. These results showed AV and EF may serve as potentially effective and safe norovirus inhibitors due to their relatively low cytotoxicity, which allows them to be prepared as food-safe formulations. (C) 2016 Elsevier Ltd. All rights reserved.