Demonstration of antigenic and genotypic variation in Orientia tsutsugamushi which were isolated in Japan, and their classification into type and subtype
Demonstration of antigenic and genotypic variation in Orientia tsutsugamushi which were isolated in Japan, and their classification into type and subtype
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DOI：
10.1111/j.1348-0421.1996.tb01120.x
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发表时间：
1996-01-01
影响因子：
2.6
通讯作者：
Yoshimura, K
中科院分区：
文献类型：
--
作者：
Ohashi, N;Koyama, Y;Yoshimura, K
共40株恙虫病东方体（34株来自日本的患者和恙螨，6株抗原变体的原型菌株）进行了检查分类的基础上的反应性与型特异性单克隆抗体在间接免疫荧光试验，和限制性片段长度多态性的聚合酶链反应（PCR）扩增的56千道尔顿型特异性抗原蛋白基因。通过这些方法，在菌株中发现了一些抗原和基因型变异，并将这些变异分为类型和亚型。这些结果表明O.恙虫病，和这里使用的方法似乎是有用的系统分类的众多变种。本研究还发现了一株可能是与以前鉴定的菌株不同的新类型的菌株。此外，还观察到与类型和/或亚型分类有关的小鼠致病性程度的变化。
A total of 40 strains of Orientia tsutsugamushi (34 isolates from patients and trombiculid mites in Japan, and 6 prototype strains of antigenic variants) were examined for classification based on the reactivities with type-specific monoclonal antibodies in indirect immunofluorescence tests, and on the restriction fragment length polymorphism of a polymerase chain reaction (PCR)-amplified 56-kilodalton type-specific antigenic protein gene. By these methods, several antigenic and genotypic variants were found among the strains, and these variants were classified into types and further into subtypes. These results suggest that there are many variants in O. tsutsugamushi, and the methods used here seem to be useful for the systematic classification of the numerous variants. A strain which may be a new type distinguishable from those identified previously was also found in this study, Furthermore, variety in the degree of pathogenicity in mice related to type and/or subtype classification were observed.