Size separation of supermicrometer particles in asymmetrical flow field-flow fractionation. Flow conditions for rapid elution.
Size separation of supermicrometer particles in asymmetrical flow field-flow fractionation. Flow conditions for rapid elution.
复制标题
非对称流场流分级中超微米颗粒的尺寸分离。
DOI：
10.1021/ac020315s
复制
发表时间：
2002
影响因子：
7.4
通讯作者：
A. Zattoni
中科院分区：
文献类型：
--
作者：
K. Wahlund;A. Zattoni
通过分离直径为2~20微米的标准聚苯乙烯乳胶粒，考察了快速洗脱条件下升力-上层非对称流动-流动分离的性能。研究了流量的优化，不仅是为了获得高效、快速的分离，而且是为了在不同形状和陡度的轴向流动速度梯度下工作。在极端流动条件下，20.5微米、15.0微米、9.7微米、5.0微米和2.0微米五种大小的颗粒可以在6分钟内分辨出来，而对于20.5-5.0微米的较窄的尺寸范围，1分钟就足够了。研究了粒径在9.7-2.0微米范围内的尺寸选择性与流量和颗粒大小的关系，发现其是恒定的。颗粒捕捉器使分离10微米大小的颗粒成为可能，这在以前被证明在不对称的通道中是困难的。
The performance of lift-hyperlayer asymmetrical flow field-flow fractionation using rapid elution conditions was tested through the separation of standard polystyrene latex particles of diameters from 2 to 20 microm. Optimization of flowrates was studied not only in order to obtain efficient and rapid separation, but also to work under conditions of various shape and steepness of the axial flow velocity gradient. Using extreme flow conditions, the five widely spaced particle sizes, 20.5-, 15.0-, 9.7-, 5.0-, and 2.0-microm diameter, could be resolved in 6 min, whereas for the narrower size range of 20.5-5.0 microm, 1 min was enough. The size selectivity in the size range 9.7-2.0 microm was studied as a function of flowrates and particle size and was found to be constant. A particle trapping device made it possible to separate particles of sizes > 10 microm, which has previously proven to be difficult in asymmetrical channels.