Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U
Genetic analyses and molecular characterization of the pathways involved in the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid in Pseudomonas putida U
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DOI：
10.1111/j.1462-2920.2007.01464.x
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发表时间：
2008-02-01
影响因子：
5.1
通讯作者：
Luengo, Jose M.
中科院分区：
文献类型：
--
作者：
Arias, Sagrario;Olivera, Elias R.;Luengo, Jose M.
在恶臭假单胞菌 U 中，2-苯乙胺和 2-苯乙醇转化为苯乙酸需要两种不同的途径（Pea、Ped）。 2-苯乙胺途径 (PeaABCDEFGHR) 催化该胺的转运、通过醌血红蛋白胺脱氢酶将其脱氨基为苯乙醛，以及通过苯乙醛脱氢酶催化的反应将该化合物氧化。 2-苯基乙醇的摄取以及通过苯乙醛氧化为苯乙酸需要另一种分解代谢途径（PedS(1)R(1)ABCS(2)R(2)DEFGHI）。这意味着两种不同的双组分信号转导系统、两种醌蛋白醇脱氢酶、细胞色素c、周质结合蛋白、醛脱氢酶、五肽重复蛋白和ABC外排系统的参与。此外，两个辅助元件组（PqqABCDEF 和 CcmABCDEFGHI）对于主要通路（Pea 和 Ped）的运行是必需的。 PqqABCDEF 是吡咯喹啉醌 (PQQ) 生物合成所必需的，PQQ 是某些醇脱氢酶的辅基，可将电子转移到独立的细胞色素 c；而 CcmABCDEFGHI 是细胞色素 c 成熟所必需的。我们的数据表明，恶臭假单胞菌 U 中苯乙胺和苯乙醇的降解与大肠杆菌中报道的降解有很大不同，并且证明 PeaABCDEFGHR 和 PedS(1)R(1)ABCS(2)R(2)DEFGHI 是属于苯乙酰辅酶 A 分解代谢物的两条上游路径。
In Pseudomonas putida U two different pathways (Pea, Ped) are required for the conversion of 2-phenylethylamine and 2-phenylethanol into phenylacetic acid. The 2-phenylethylamine pathway (PeaABCDEFGHR) catalyses the transport of this amine, its deamination to phenylacetaldehyde by a quinohaemoprotein amine dehydrogenase and the oxidation of this compound through a reaction catalysed by a phenylacetaldehyde dehydrogenase. Another catabolic route (PedS(1)R(1)ABCS(2)R(2)DEFGHI) is needed for the uptake of 2-phenylethanol and for its oxidation to phenylacetic acid via phenylacetaldehyde. This implies the participation of two different two-component signal-transducing systems, two quinoprotein alcohol dehydrogenases, a cytochrome c, a periplasmic binding protein, an aldehyde dehydrogenase, a pentapeptide repeat protein and an ABC efflux system. Additionally, two accessory sets of elements (PqqABCDEF and CcmABCDEFGHI) are necessary for the operation of the main pathways (Pea and Ped). PqqABCDEF is required for the biosynthesis of pyrroloquinoline quinone (PQQ), a prosthetic group of certain alcohol dehydrogenases that transfers electrons to an independent cytochrome c; whereas CcmABCDEFGHI is required for cytochrome c maturation. Our data show that the degradation of phenylethylamine and phenylethanol in P. putida U is quite different from that reported in Escherichia coli, and they demonstrate that PeaABCDEFGHR and PedS(1)R(1)ABCS(2)R(2)DEFGHI are two upper routes belonging to the phenylacetyl-CoA catabolon.