Correlation between Thermal Radiosensitization and Heat-induced Loss of DNA Polymerase β Activity in CHO Cells
Correlation between Thermal Radiosensitization and Heat-induced Loss of DNA Polymerase β Activity in CHO Cells
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CHO 细胞中热放射增敏与热诱导 DNA 聚合酶 β 活性丧失之间的相关性
DOI：
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发表时间：
1993
期刊：
影响因子：
--
通讯作者：
Horst Jung
中科院分区：
文献类型：
--
作者：
E. Dikomey;Horst Jung
在CHO细胞中研究了各种单一和联合热处理引起的辐射敏感性的增加，这与热诱导DNA聚合酶β活性的丧失有关。通过10%生存水平下的热增强比TER10%和细胞生存数据拟合公式- ln(S/S0) = α d + β d2得到的参数α和β来量化热辐射敏化。TER10%和α-项与聚合酶β活性呈负相关；温度≥41.5℃的单次加热和耐热细胞的数据呈同一直线下降，而温度≥41.5℃的单次加热和从高到低的顺序加热（降压加热）细胞的TER10%和α的增加幅度比其他热处理要大得多。相反，细胞放射敏感性的β项与聚合酶β活性的丧失呈线性关系。
The increase in radiosensitivity caused by various kinds of single and combined heat treatments was studied in CHO cells and related to the heat-induced loss of the DNA polymerase β activity. Thermal radiosensitization was quantified by the thermal enhancement ratio TER10% determined on the 10% survival level and by the parameters α and β obtained from fitting cellular survival data to the equation − ln(S/S0) = αD + βD2. The values for TER10% and the α-term showed only a poor correlation with the inverse of polymerase β activity; the data for single heating at temperatures exceeding 41·5°C and the data for thermotolerant cells fell on the same straight line, whereas for single heating at T ⩽ 41·5°C and for cells exposed to a high-to-low temperature sequence (step-down heating) the increase in TER10% and α was much steeper than found for the other heat treatments. By contrast, a linear relationship was shown to exist between the β-term of cellular radiosensitivity and the loss of polymerase β activity as e...
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影响因子：
3.4
作者：
CURTIS, SB
通讯作者：
CURTIS, SB
核基质蛋白质量的变化与热诱导的 DNA 单链断裂修复抑制相关。
DOI：
10.1080/09553008714551761
发表时间：
1987
期刊：
International journal of radiation biology and related studies in physics, chemistry, and medicine
影响因子：
--
作者：
Warters,RL;Brizgys,LM;Lyons,BW
通讯作者：
Lyons,BW
CHO 细胞从高温增强到 DNA 和染色质 X 射线修复的恢复。
影响因子：
3.4
作者：
Clark,EP;Dewey,WC;Lett,JT
通讯作者：
Lett,JT
放线菌酮对中国仓鼠卵巢细胞热诱导细胞杀伤、放射增敏和细胞 DNA 聚合酶活性丧失的影响。
影响因子：
3.4
作者：
Chu,GL;Dewey,WC
通讯作者：
Dewey,WC
影响因子：
2.6
作者：
THAMES, HD
通讯作者：
THAMES, HD