RODENT STROKE MODEL GUIDELINES FOR PRECLINICAL STROKE TRIALS (1ST EDITION).
RODENT STROKE MODEL GUIDELINES FOR PRECLINICAL STROKE TRIALS (1ST EDITION).
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DOI：
10.6030/1939-067x-2.2.2
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发表时间：
2009-01-01
期刊：
影响因子：
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通讯作者：
Winn HR
中科院分区：
文献类型：
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作者：
Liu S;Zhen G;Meloni BP;Campbell K;Winn HR
翻译性卒中研究是一项具有挑战性的任务，需要卒中研究界的长期团队合作。具有良好结果一致性的高度可重复性的卒中模型对于从临床前卒中试验中获得有用的数据以及提高实验室间的可比性是必不可少的。然而，我们的文献回顾表明，常用的卒中模型的梗塞变异系数在5%到200%之间。对常用卒中模型的全面改进将进一步提高实验性卒中研究的质量以及实验室间的可比性。许多因素在导致结果差异方面发挥了重要作用；然而，在卒中治疗学术行业圆桌会议(STAIR)建议和良好实验室实践(GLP)中，这些因素尚未得到充分解决。这些关键因素包括麻醉药的选择、动物生理环境的维持、卒中结果的观察以及影响成功率和变异的模型特异性因素。作者综述了这些已报道的影响卒中模型结果的主要因素，在此提供第一版卒中模型指南，以启动对这一主题的积极讨论。我们希望在不久的将来，通过它的连续更新版本，在中风研究人员之间达成普遍的共识。
Translational stroke research is a challenging task that needs long term team work of the stroke research community. Highly reproducible stroke models with excellent outcome consistence are essential for obtaining useful data from preclinical stroke trials as well as for improving inter-lab comparability. However, our review of literature shows that the infarct variation coefficient of commonly performed stroke models ranges from 5% to 200%. An overall improvement of the commonly used stroke models will further improve the quality for experimental stroke research as well as inter-lab comparability. Many factors play a significant role in causing outcome variation; however, they have not yet been adequately addressed in the Stroke Therapy Academic Industry Roundtable (STAIR) recommendations and the Good Laboratory Practice (GLP). These critical factors include selection of anesthetics, maintenance of animal physiological environment, stroke outcome observation, and model specific factors that affect success rate and variation. The authors have reviewed these major factors that have been reported to influence stroke model outcome, herewith, provide the first edition of stroke model guidelines so to initiate active discussion on this topic. We hope to reach a general agreement among stroke researchers in the near future with its successive updated versions.