MEASUREMENT OF PHENYLALANINE IN ROUTINE CARE OF PHENYLKETONURIC CHILDREN
MEASUREMENT OF PHENYLALANINE IN ROUTINE CARE OF PHENYLKETONURIC CHILDREN
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DOI：
10.1136/adc.48.6.472
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发表时间：
1973-01-01
影响因子：
5.2
通讯作者：
FARQUHAR, JW
中科院分区：
文献类型：
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作者：
BELTON, NR;CROMBIE, JD;FARQUHAR, JW
荧光测定法、纸色谱和柱色谱法以及细菌抑制测定法对血清或血液苯丙氨酸的定量进行了比较，苯丙氨酸的浓度范围为 1 至超过 30 mg/100 ml。特别注意 2 mg 或更少的水平以及超过 20 mg/100 ml 的水平，因为这些水平具有治疗意义。在常规条件下，细菌抑制测定在 2 mg 或以下时往往读数较低或根本没有读数，而在 20 mg/100 ml 以上读数则较高，因此在这种条件下“安全失败”。纸色谱法被证明是一种简单、快速且经济的方法，可以在检查范围内的各个级别获得准确的结果。
Fluorimetry, paper and column chromatography, and bacterial inhibition assay have been compared in the quantitation of serum or blood phenylalanine at levels ranging from 1 to over 30 mg/100 ml. Special attention was paid to levels of 2 mg or less and to those in excess of 20 mg/100 ml, since these have therapeutic implications. Bacterial inhibition assay, under routine conditions, tended to read low or not at all at 2 mg or less, and to read high above 20 mg/100 ml so that it `failed safe' under such conditions. Paper chromatography was shown to be a simple, rapid, and economic method of getting accurate results at all levels in the range examined.